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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.12
- 수록면
- 189 - 197 (9page)
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Purpose: Cell therapy for various diseases has gained wide acceptance. Because most patients with chronic liver failure have mild-to-severe liver cirrhosis, there are many limitations to clinical applications. We analyzed how to increase cell engraftment in rats with liver fibrosis.
Methods: We used analbuminemic SD rats (NARs) as recipients of syngeneic CAG-EGFP SD hepatocytes obtained by the 2 perfusion method. Hepatic fibrosis was induced with thioacetamide in drinking water for 6 weeks in the recipient NARs. NARs were pre-treated with gadolinium, doxorubicin, and gliotoxin before hepatocyte transplantation. We evaluated the degree of cell engraftment by RT-PCR and immunofluorescent staining for GFP and albumin. The transplanted cells were detected by immunostaining for albumin, and serum albumin was also measured.
Results: Although detection of GFP by RT-PCR was variable, albumin was detected in all groups 4 wks after hepatocyte transplantation. GFP and albumin were also detected by immunofluorescent staining 1 and 4 wks after cell transplantation. In control rats, albumin production was maximal at 3 wks, and after that it rapidly decreased. In the gadolinium and doxorubicin-treated group, albumin production was increased up to 4 wks. Albumin production in the gadolinium-treated group was superior to that of the doxorubicin-treated group.
Conclusion: Kupffer cells play the most important role in cell engraftment in hepatic fibrosis. Therefore, perturbation of kupffer cells in hepatic fibrosis is needed to increase cell engraftment.
Methods: We used analbuminemic SD rats (NARs) as recipients of syngeneic CAG-EGFP SD hepatocytes obtained by the 2 perfusion method. Hepatic fibrosis was induced with thioacetamide in drinking water for 6 weeks in the recipient NARs. NARs were pre-treated with gadolinium, doxorubicin, and gliotoxin before hepatocyte transplantation. We evaluated the degree of cell engraftment by RT-PCR and immunofluorescent staining for GFP and albumin. The transplanted cells were detected by immunostaining for albumin, and serum albumin was also measured.
Results: Although detection of GFP by RT-PCR was variable, albumin was detected in all groups 4 wks after hepatocyte transplantation. GFP and albumin were also detected by immunofluorescent staining 1 and 4 wks after cell transplantation. In control rats, albumin production was maximal at 3 wks, and after that it rapidly decreased. In the gadolinium and doxorubicin-treated group, albumin production was increased up to 4 wks. Albumin production in the gadolinium-treated group was superior to that of the doxorubicin-treated group.
Conclusion: Kupffer cells play the most important role in cell engraftment in hepatic fibrosis. Therefore, perturbation of kupffer cells in hepatic fibrosis is needed to increase cell engraftment.
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참고문헌
참고문헌 (30)
참고문헌 신청
1. [기타자료] - Korean Statistical Information Service / / The statistical Results of causes of death in 2008
2. [학술지(정기간행물)] - 정숙향 / 2008 / 국내 A형간염의 현황과 백신 적응증 / 대한소화기학회지 51 (6) : 331 ~ 337
3. [학술지(정기간행물)] - Gotthardt D / 2007 / Fulminant hepatic failure: etiology and indications for liver transplantation / Nephrol Dial Transplant 22 (8) : viii5 ~ viii8
4. [학술지(정기간행물)] - Lidofsky SD / 1993 / Liver transplantation for fulminant hepatic failure / Gastroenterol Clin North Am 22 : 257 ~ 269
5. [학술지(정기간행물)] - Starzl TE / 1989 / Liver transplantation (2) / N Engl J Med 321 : 1092 ~ 1096
2. [학술지(정기간행물)] - 정숙향 / 2008 / 국내 A형간염의 현황과 백신 적응증 / 대한소화기학회지 51 (6) : 331 ~ 337
3. [학술지(정기간행물)] - Gotthardt D / 2007 / Fulminant hepatic failure: etiology and indications for liver transplantation / Nephrol Dial Transplant 22 (8) : viii5 ~ viii8
4. [학술지(정기간행물)] - Lidofsky SD / 1993 / Liver transplantation for fulminant hepatic failure / Gastroenterol Clin North Am 22 : 257 ~ 269
5. [학술지(정기간행물)] - Starzl TE / 1989 / Liver transplantation (2) / N Engl J Med 321 : 1092 ~ 1096
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