The main purpose of this study was to investigate bilateral muscle imbalance based on isokinetic muscle strength in knee and muscle fatigue in elite cyclists observed by electromyographic analysis measured on Wingate test. In doing so, we also found out the corelationship between isokinetic muscle strength and pedaling power. Six elite cyclists were recruited and isokinetic knee and lumbar extension and flexion were measured at 60 degree per sec and muscle fatigue pattern during Wingate test was analyzed to see if any muscle asymetry exists between dominant and non-dominant muscle strength. As a result paired t-test, there was no difference in muscle strength in knee extension and flexion between dominant and non-dominant even though subtle difference in terms of Limb Symetry Index (LSI) was observed. No difference in muscle fatigue pattern in dominant and non-dominant leg observed in Wingate test. Therefore, cyclists involved in cyclic exercise in which right and left limb are relatively uniformly recruited tend to have less or little muscle asymetry between dominant and non-dominant limb.