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논문 기본 정보

자료유형
학술저널
저자정보
Myat Htut Nyunt (Kangwon National University) Myat Phone Kyaw (Department of Medical Research) Kyaw Zin Thant (Department of Medical Research) Thinzer Shein (Department of Medical Research) Soe Soe Han (Department of Medical Research) Ni Ni Zaw (Department of Medical Research) Jin-Hee Han (Kangwon National University) Seong-Kyun Lee (Kangwon National University) Fauzi Muh (Kangwon National University) Jung-Yeon Kim (Centers for Disease Control and Prevention) Shin-Hyeong Cho (Centers for Disease Control and Prevention) Sang-Eun Lee (Centers for Disease Control and Prevention) Eun-Jeong Yang (Centers for Disease Control and Prevention) Chulhun L. Chang (Pusan National University Yangsan Hospital) Eun-Taek Han (Kangwon National University)
저널정보
대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.54 No.3
발행연도
2016.6
수록면
253 - 259 (7page)

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In the era of (pre) elimination setting, the prevalence of malaria has been decreasing in most of the previously endemic areas. Therefore, effective cost- and time-saving validated pooling strategy is needed for detection of malaria in low transmission settings. In this study, optimal pooling numbers and lowest detection limit were assessed using known density samples prepared systematically, followed by genomic DNA extraction and nested PCR. Pooling strategy that composed of 10 samples in 1 pool, 20 μl in 1 sample, was optimal, and the parasite density as low as 2 p/μl for both falciparum and vivax infection was enough for detection of malaria. This pooling method showed effectiveness for handling of a huge number of samples in low transmission settings (<9% positive rate). The results indicated that pooling of the blood samples before DNA extraction followed by usual nested PCR is useful and effective for detection of malaria in screening of hidden cases in low-transmission settings.

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Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

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UCI(KEPA) : I410-ECN-0101-2017-513-000776031