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[학술저널]

  • 학술저널

Carl Angelo Medriano Kartik Chandran Samir Khanal Jae Woo Lee Sungpyo Kim

UCI(KEPA) : I410-ECN-0101-2018-505-001077900

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This study aims to investigate the effects of OTC in the response of a Nitrosomonas europaea, a model nitrifying bacterium. For this aim, the variations of nitrogen species in aquaculture water samples and Nitrosomonas europaea culture, as a function of OTC concentration, have been monitored. As a result, aquaculture samples show the nitrification level is lower by 23% from 5ppm, 43% from 50ppm, and 46% from 100ppm OTC as compared to a control (no added OTC). Also, the addition of 50ppm OTC to Nitrosomonas europaea culture showed 29% lower degradation level compared to a control. As compared to control, the 10ppm OTC showed increased nitrite level by 30% and nitrous oxide level by up to 29% after 80 hrs. RNA expression analysis was able correlate amoA expression to the reduced level of ammonia removal. The hao, nirK, and norB gene expressions did not show correlation to the oxytetracycline concentration, thus suggesting that the dose influenced in other ways perhaps somewhere during post-transcription to enzyme activity

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