Broccoli (Brassica oleracea) Reduces Oxidative Damage to Pancreatic Tissue and Combats Hyperglycaemia in Diabetic Rats

Sithara Suresh; Mostafa Ibrahim Waly; Mohammad Shafiur Rahman; Nejib Guizani; Mohamed Abdullah Badar Al-Kindi; Halima Khalfan Ahmed Al-Issaei; Sultan Nasser Mohd Al-Maskari; Bader Rashid Said Al-Ruqaishi; Ahmed Al-Salami

doi:10.3746/pnf.2017.22.4.277

추천

검색

질문

자료유형: 학술저널

저자정보: Sithara Suresh (Sultan Qaboos University) Mostafa Ibrahim Waly (Sultan Qaboos University) Mohammad Shafiur Rahman (Sultan Qaboos University) Nejib Guizani (Sultan Qaboos University) Mohamed Abdullah Badar Al-Kindi (Sultan Qaboos University) Halima Khalfan Ahmed Al-Issaei (Sultan Qaboos University) Sultan Nasser Mohd Al-Maskari (Sultan Qaboos University) Bader Rashid Said Al-Ruqaishi (Sultan Qaboos University) Ahmed Al-Salami (Ministry of Health)

저널정보: 한국식품영양과학회 Preventive Nutrition and Food Science Preventive Nutrition and Food Science Vol.22 No.4

발행연도: 2017.12

수록면: 277 - 284 (8page)

DOI: 10.3746/pnf.2017.22.4.277

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Oxidative stress plays a pivotal role in the development of diabetes and hyperglycaemia. The protective effects of natural extracts against diabetes are mainly dependent on their antioxidant and hypoglycaemic properties. Broccoli (Brassica oleracea) exerts beneficial health effects in several diseases including diabetes; however, the mechanism has not been elucidated yet. The present study was carried out to evaluate the potential hypoglycaemic and antioxidant properties of aqueous broccoli extracts (BEs) in diabetic rats. Streptozotocin (STZ) drug was used as a diabetogenic agent in a single intraperitoneal injection dose of 50 mg/kg body weight. The blood glucose level for each rat was measured twice a week. After 8 weeks, all animals were fasted overnight and sacrificed; pancreatic tissues were homogenized and used for measuring oxidative DNA damage, biochemical assessment of glutathione (GSH), and total antioxidant capacity (TAC) as well as histopathological examination for pancreatic tissues was examined. Diabetic rats showed significantly higher levels of DNA damage, GSH depletion, and impaired TAC levels in comparison to non-diabetics (P<0.05). The treatment of diabetic rats with BE significantly reduced DNA damage and conserved GSH and TAC values (P<0.01). BE attenuated pancreatic histopathological changes in diabetic rats. The results of this study indicated that BE reduced the STZ mediated hyperglycaemia and the STZ-induced oxidative injury to pancreas tissue. The used in vivo model confirmed the efficacy of BE as an anti-diabetic herbal medicine and provided insights into the capacity of BE to be used for phytoremediation purposes for human type 2 diabetes.

#broccoli extract #oxidative stress #type 2 diabetes #hyperglycaemia #streptozotocin

참고문헌 (40)

참고문헌 신청

Lee SG, Kim JH, Son MJ, Lee EJ, Park WD, Kim JB, Lee SP, Lee IS. 2013. Influence of extraction method on quality and functionality of broccoli juice. Prev Nutr Food Sci 18: 133-138. El-Magd MMA. 2013. Evaluation of some broccoli cultivars growth, head yield and quality under different planting dates. J Appl Sci Res 9: 5730-5736. Ares AM, Nozal MJ, Bernal J. 2013. Extraction, chemical characterization and biological activity determination of broccoli health promoting compounds. J Chromatogr A 1313: 78-95. Moreno DA, Carvajal M, Lopez-Berenguer C, Garcia-Viguera C. 2006. Chemical and biological characterisation of nutraceutical compounds of broccoli. J Pharm Biomed Anal 41: 1508- 1522. Campbell B, Han D, Triggs CM, Fraser AG, Ferguson LR. 2012. Brassicaceae: nutrient analysis and investigation of tolerability in people with Crohn’s disease in a New Zealand study. Funct Foods Health Dis 2: 460-486.

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!