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[학술저널]

  • 학술저널

양아여 조정용 박양균

UCI(KEPA) : I410-ECN-0101-2018-059-003065707

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초록

The antioxidant substance in black onion was identified. The assays that used 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2`-azino-bis(3-ethylbenzthiazo line-6-sulphonic acid) (ABTS+) radicals showed that the ethyl acetate fraction of black onion methanol extract had a higher level of radical-scavenning activity than the other fractions. Two antioxidative compounds were purified and isolated from the ethyl acetate fraction via column chromatographies of silica gel and Sephadex LH-20 using the guided DPPH radical-scavenging assay. The isolated compounds were identified as 3,4-dihydroxybenzoic acid (1) and quercetin (2) based on mass spectrometry and nuclear magnetic resonance. The isolated compounds showed a high level of DPPH and ABTS+ radical-scavenging activity. Compound 2 had a higher level of radical-scavenging activity than 1.

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