이 연구에서는 GC-MS를 사용하여 신선편의 채소류 중 DCAA 및 TCAA를 포함한 haloacetic acids를 분석하는 방법을 확립하였다. 분석 방법의 유효성을 검증한 후 일부 시중에서 유통 중인 시료를 분석한 결과, 외국의 수준보다 높아 19-75 μg/kg의 수준으로 검출되었다. 본 결과를 바탕으로 실시한 위해 평가에 따르면, 현재 국내에 유통 중인 신선편의 채소류의 섭취에 따른 초과 발암 위해도가 허용 수준을 초과하여, 향후 이에 대한 관리가 필요하다는 것을 시사하였다.
Dichloroacetic acid (DCAA) and trichloroacetic acid (TCAA) in fresh-cut salads available from the market were determined by gas chromatography-mass spectrometry (GC-MS). The target compounds in 3 g of acidified homogenates were extracted with 20 mL of methyl t-butyl ether (MTBE). The extract was concentrated to 1 mL and heated for 1 h at 55℃. The analytes were separated using a DB-1701 column and detected with a mass spectrometer. The method detection limit was approximately 6 μg/kg, and both analytical accuracy and precision were found to be satisfactory. The linearity of the calibration curves expressed as the coefficients of determination was >0.996. The analysis of seven samples using the established method showed that the four samples contained considerable amounts of analytes (25.4-31.2 μg/kg of DCAA and 18.8-46.1 μg/kg of TCAA). These results raised a concern about the impact of fresh-cut salad consumption on human health.