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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.4
- 수록면
- 179 - 185 (7page)
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초록· 키워드
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Background: Oxidative stress induces the production of reactive oxygen species (ROS), which play important causative roles in various pathological conditions. Black ginseng (BG), a type of steamprocessed ginseng, has drawn significant attention due to its biological activity, and is more potent than white ginseng (WG) or red ginseng (RG).
Methods: We evaluated the protective effects of BG extract (BGE) against oxidative stress-induced cellular damage, in comparison with WG extract (WGE) and RG extract (RGE) in a cell culture model. Ethanolic extracts of WG, RG, and BG were used to evaluate ginsenoside profiles, total polyphenols, flavonoid contents, and antioxidant activity. Using AML-12 cells treated with H₂O₂, the protective effects of WGE, RGE, and BGE on cellular redox status, DNA, protein, lipid damage, and apoptosis levels were investigated.
Results: BGE exhibited significantly enhanced antioxidant potential, as well as total flavonoid and polyphenol contents. ATP levels were significantly higher in BGE-treated cells than in control; ROS generation and glutathione disulfide levels were lower but glutathione (GSH) and NADPH levels were higher in BGE-treated cells than in other groups. Pretreatment with BGE inhibited apoptosis and therefore protected cells from oxidative stress-induced cellular damage, probably through ROS scavenging.
Conclusion: Collectively, our results demonstrate that BGE protects AML-12 cells from oxidative stressinduced cellular damages more effectively than WGE or RGE, through ROS scavenging, maintenance of redox status, and activation of the antioxidant defense system.
Methods: We evaluated the protective effects of BG extract (BGE) against oxidative stress-induced cellular damage, in comparison with WG extract (WGE) and RG extract (RGE) in a cell culture model. Ethanolic extracts of WG, RG, and BG were used to evaluate ginsenoside profiles, total polyphenols, flavonoid contents, and antioxidant activity. Using AML-12 cells treated with H₂O₂, the protective effects of WGE, RGE, and BGE on cellular redox status, DNA, protein, lipid damage, and apoptosis levels were investigated.
Results: BGE exhibited significantly enhanced antioxidant potential, as well as total flavonoid and polyphenol contents. ATP levels were significantly higher in BGE-treated cells than in control; ROS generation and glutathione disulfide levels were lower but glutathione (GSH) and NADPH levels were higher in BGE-treated cells than in other groups. Pretreatment with BGE inhibited apoptosis and therefore protected cells from oxidative stress-induced cellular damage, probably through ROS scavenging.
Conclusion: Collectively, our results demonstrate that BGE protects AML-12 cells from oxidative stressinduced cellular damages more effectively than WGE or RGE, through ROS scavenging, maintenance of redox status, and activation of the antioxidant defense system.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results
4. Discussion
References
참고문헌 (34)
참고문헌 신청
Schieber M / 2014 / ROS function in redox signaling and oxidative stress / Curr Biol 24:R453~R462
Reuter S / 2010 / Oxidative stress, inflammation, and cancer : how are they linked / Free Radical Biol Med 49:1603~1616
Mates JM / 2000 / Effects of antioxidant enzymes in the molecular control of reactive oxygen species toxicology / Toxicology 153:83~104
Islam MT / 2017 / Oxidative stress and mitochondrial dysfunction-linked neurodegenerative disorders / Neurol Res 39:73~82
Mondal NK / 2016 / Increased risk of cardiovascular disease in premenopausal female ragpickers of Eastern India : involvement of inflammation, oxidative stress, and platelet hyperactivity / Mol Cell Biochem 419:193~203
Reuter S / 2010 / Oxidative stress, inflammation, and cancer : how are they linked / Free Radical Biol Med 49:1603~1616
Mates JM / 2000 / Effects of antioxidant enzymes in the molecular control of reactive oxygen species toxicology / Toxicology 153:83~104
Islam MT / 2017 / Oxidative stress and mitochondrial dysfunction-linked neurodegenerative disorders / Neurol Res 39:73~82
Mondal NK / 2016 / Increased risk of cardiovascular disease in premenopausal female ragpickers of Eastern India : involvement of inflammation, oxidative stress, and platelet hyperactivity / Mol Cell Biochem 419:193~203
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