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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.4
- 수록면
- 305 - 311 (7page)
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초록· 키워드
오류제보하기
Background: Gintonin is a ginseng-derived exogenous ligand of the G protein-coupled lysophosphatidic acid (LPA) receptor. We previously reported that gintonin stimulates gliotransmitter release in primary cortical astrocytes. Astrocytes play key roles in the functions of neurovascular systems. Although vascular endothelial growth factor (VEGF) is known to influence the normal growth and maintenance of cranial blood vessels and the nervous system, there is little information about the effect of gintonin on VEGF regulation in primary astrocytes, under normal and hypoxic conditions.
Methods: Using primary cortical astrocytes of mice, the effects of gintonin on the release, expression, and distribution of VEGF were examined. We further investigated whether the gintonin-mediated VEGF release protects astrocytes from hypoxia.
Results: Gintonin administration stimulated the release and expression of VEGF from astrocytes in a concentration- and time-dependent manner. The gintonin-mediated increase in the release of VEGF was inhibited by the LPA1/3 receptor antagonist, Ki16425; phospholipase C inhibitor, U73122; inositol 1,4,5-triphosphate receptor antagonist, 2-APB; and intracellular Ca<SUP>2+</SUP> chelator, BAPTA. Hypoxia further stimulated astrocytic VEGF release. Gintonin treatment stimulated additional VEGF release and restored cell viability that had decreased due to hypoxia, via the VEGF receptor pathway. Altogether, the regulation of VEGF release and expression and astrocytic protection mediated by gintonin under hypoxia are achieved via the LPA receptoreVEGF signaling pathways.
Conclusion: The present study shows that the gintonin-mediated regulation of VEGF in cortical astrocytes might be neuroprotective against hypoxic insults and could explain the molecular basis of the beneficial effects of ginseng on the central nervous system.
Methods: Using primary cortical astrocytes of mice, the effects of gintonin on the release, expression, and distribution of VEGF were examined. We further investigated whether the gintonin-mediated VEGF release protects astrocytes from hypoxia.
Results: Gintonin administration stimulated the release and expression of VEGF from astrocytes in a concentration- and time-dependent manner. The gintonin-mediated increase in the release of VEGF was inhibited by the LPA1/3 receptor antagonist, Ki16425; phospholipase C inhibitor, U73122; inositol 1,4,5-triphosphate receptor antagonist, 2-APB; and intracellular Ca<SUP>2+</SUP> chelator, BAPTA. Hypoxia further stimulated astrocytic VEGF release. Gintonin treatment stimulated additional VEGF release and restored cell viability that had decreased due to hypoxia, via the VEGF receptor pathway. Altogether, the regulation of VEGF release and expression and astrocytic protection mediated by gintonin under hypoxia are achieved via the LPA receptoreVEGF signaling pathways.
Conclusion: The present study shows that the gintonin-mediated regulation of VEGF in cortical astrocytes might be neuroprotective against hypoxic insults and could explain the molecular basis of the beneficial effects of ginseng on the central nervous system.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results
4. Discussion
References
참고문헌 (31)
참고문헌 신청
Pagès C / 2001 / Lysophosphatidic acid synthesis and release / Prostagl Other Lipid Mediat 64:1~10
Yung YC / 2015 / Lysophosphatidic acid signaling in the nervous system / Neuron 85:669~682
Araque A / 1999 / Tripartite synapses : glia, the unacknowledged partner / Trends in Neuroscience 22:208~215
Halassa MM / 2007 / The tripartite synapse : roles for gliotransmission in health and disease / Trends Mol Med 13:54~63
Shano S / 2008 / Lysophosphatidic acid stimulates astrocyte proliferation through LPA1 / Neurochem Int 52:216~220
Yung YC / 2015 / Lysophosphatidic acid signaling in the nervous system / Neuron 85:669~682
Araque A / 1999 / Tripartite synapses : glia, the unacknowledged partner / Trends in Neuroscience 22:208~215
Halassa MM / 2007 / The tripartite synapse : roles for gliotransmission in health and disease / Trends Mol Med 13:54~63
Shano S / 2008 / Lysophosphatidic acid stimulates astrocyte proliferation through LPA1 / Neurochem Int 52:216~220
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