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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 168 - 179 (12page)
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We previously identified the rice gene, OsSAP, as an encoder of a highly conserved putative senescenceassociated protein that was shown to have anti-apoptotic activity. To confirm the role of OsSAP in inducing abiotic stress tolerance in rice, we introduced OsSAP and AtBI-1, a plant homologue of Bax inhibitor-1, under the control of the CaMV 35S promoter into the rice genome through Agrobacterium- mediated transformation. The OsSAP transformants showed a similar chlorophyll index after salinity treatments with AtBI-1. Furthermore, we compared the effects of salinity stress on leaves and roots by examining the hormone levels of abscisic acid (ABA), jasmonic acid (JA), gibberellic acid (GA3), and zeatin in transformants compared to the control. With the exception of phytohormones, stress-induced changes in hormone levels putatively related to stress tolerance have not been investigated previously. Hormonal level analysis confirmed the lower rate of stress in the transformants compared to the control. The levels of ABA and JA in OsSAP and AtBI-1 transformants were similar, where stress rates increased after one week and decreased after a two week period of drought; there was a slightly higher accumulation compared to the control. However, a similar trend was not observed for the level of zeatin, as the decrease in the level of zeatin accumulation differed in both OsSAP and AtBI-1 transformants for all genotypes during the early period of salinity stress. The GA3 level was detected under normal conditions, but not under salinity stress.
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참고문헌 (39)
참고문헌 신청
Kroemer G / 1997 / Theproto-oncogene Bcl-2 and it srolein regulating apoptosis / Nat Med 3:614~620
Lacomme C / 1999 / Bax-induced cell death in tobacco is similar to hypersensitive response / Proc Natl Acad Sci USA 96:7956~7961
Lehmann J / 1995 / Accumulation of Jasmonate, abscisic acid, specific transcripts and proteins in osmotically stressed barley leaf segments / Planta 197:156~162
Munns R / 2002 / Comparative physiology of salt and water stress / Plant, Cell and Environment 25:239~250
Munns R / 2005 / Genes and salt tolerance:bringing them together / New phytol 167:645~663
Lacomme C / 1999 / Bax-induced cell death in tobacco is similar to hypersensitive response / Proc Natl Acad Sci USA 96:7956~7961
Lehmann J / 1995 / Accumulation of Jasmonate, abscisic acid, specific transcripts and proteins in osmotically stressed barley leaf segments / Planta 197:156~162
Munns R / 2002 / Comparative physiology of salt and water stress / Plant, Cell and Environment 25:239~250
Munns R / 2005 / Genes and salt tolerance:bringing them together / New phytol 167:645~663
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