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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한치과보철학회 The Journal of Advanced of Prosthodontics The Journal of Advanced of Prosthodontics 제7권 제6호
- 발행연도
- 2015.1
- 수록면
- 496 - 505 (10page)
이용수
초록· 키워드
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PURPOSE. To determine the effect of fibronectin (FN)-conjugated, microgrooved titanium (Ti) on osteoblast differentiation and gene expression in human bone marrow−derived mesenchymal stem cells (MSCs). MATERIALS AND METHODS. Photolithography was used to fabricate the microgrooved Ti, and amine functionalization (silanization) was used to immobilize fibronectin on the titanium surfaces. Osteoblast differentiation and osteoblast marker gene expression were analyzed by means of alkaline phosphatase activity assay, extracellular calcium deposition assay, and quantitative real-time PCR. RESULTS. The conjugation of fibronectin on Ti significantly increased osteoblast differentiation in MSCs compared with non-conjugated Ti substrates. On the extracellular calcium deposition assays of MSCs at 21 days, an approximately two-fold increase in calcium concentration was observed on the etched 60-μm-wide/10-μm-deep microgrooved surface with fibronectin (E60/10FN) compared with the same surface without fibronectin (E60/10), and a more than fourfoldincrease in calcium concentration was observed on E60/10FN compared with the non-etched control (NE0) and etched control (E0) surfaces. Through a series of analyses to determine the expression of osteoblast marker genes, a significant increase in all the marker genes except type I collagen α1 mRNA was seen with E60/10FN more than with any of the other groups, as compared with NE0. CONCLUSION. The FN-conjugated, microgrooved Ti substrate can provide an effective surface to promote osteoblast differentiation and osteoblast marker gene expression in MSCs.
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참고문헌 (23)
참고문헌 신청
Branemark PI / 1982 / Titanium implants permanently penetrating human skin / Scand J Plast Reconstr Surg 16 : 17 ~ 21
Chou L / 1995 / Substratum surface topography alters cell shape and regulates fibronectin mRNA level, mRNA stability, secretion and assembly in human fibroblasts / J Cell Sc
Dalby MJ / 2006 / Osteoprogenitor response to defined topographies with nanoscale depths / Biomaterials 27 : 1306 ~ 1315
Dean JW / 1995 / Fibronectin and laminin enhance gingival cell attachment to dental implant surfaces in vitro / Int J Oral Maxillofac Implants 10 : 721 ~ 728
Engvall E / 1977 / Binding of soluble form of fibroblast surface protein, fibronectin, to collagen / Int J Cancer 20 : 1 ~ 5
Chou L / 1995 / Substratum surface topography alters cell shape and regulates fibronectin mRNA level, mRNA stability, secretion and assembly in human fibroblasts / J Cell Sc
Dalby MJ / 2006 / Osteoprogenitor response to defined topographies with nanoscale depths / Biomaterials 27 : 1306 ~ 1315
Dean JW / 1995 / Fibronectin and laminin enhance gingival cell attachment to dental implant surfaces in vitro / Int J Oral Maxillofac Implants 10 : 721 ~ 728
Engvall E / 1977 / Binding of soluble form of fibroblast surface protein, fibronectin, to collagen / Int J Cancer 20 : 1 ~ 5
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