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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 88 - 93 (6page)
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초록· 키워드
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Dirofilaria immitis is a filarial nematode parasite that causes cardiopulmonary dirofilariasis in dogs. The purpose of this study was the development of real-time PCR assays for efficient detection of D. immitis. The D. immitis-specific primers confirmed in our previous study and a newly designed TaqMan probe were used for quantitative diagnostics. First, SYBR Green real-time PCR was performed using the specific primers and serially diluted genomic DNA or plasmid DNA, and melting curve analyses were performed after amplification. The melting curve showed one specific peak in each of the genomic and plasmid DNA reactions, suggesting that the primers specifically amplify the D. immitis cytochrome c oxidase subunit I gene. Comparison of SYBR Green and TaqMan real-time PCR using serially diluted plasmid DNA showed higher efficiency and specificity with TaqMan real-time PCR. The real-time PCR assays developed in this study will provide improved diagnostic methods to overcome the limitations of conventional diagnostic tools and facilitate more rapid and accurate diagnoses.
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참고문헌 (24)
참고문헌 신청
Watts KJ / 1999 / Development of a PCR-and probe-based test for the sensitive and specific detection of the dog heartworm, Dirofilaria immitis, in its mosquito intermediate host / Mol Cell Probes 13:425~430
Kronefeld M / 2014 / Molecular detection of Dirofilaria immitis, Dirofilaria repens and Setaria tundra in mosquitoes from Germany / Parasit Vectors 7:30
Khedri J / 2014 / Canine heartworm in southeastern of Iran with review of disease distribution / Iran J Parasitol 9:560~567
FUYAN / 2014 / Expression of translationally controlled tumor protein (TCTP) gene of Dirofilaria immitis guided by transcriptomic screening / The Korean Journal of Parasitology 52(1):21~26
Rossi MI / 2010 / Detection of Wolbachia DNA in blood from dogs infected with Dirofilaria immitis / Exp Parasitol 126:270~272
Kronefeld M / 2014 / Molecular detection of Dirofilaria immitis, Dirofilaria repens and Setaria tundra in mosquitoes from Germany / Parasit Vectors 7:30
Khedri J / 2014 / Canine heartworm in southeastern of Iran with review of disease distribution / Iran J Parasitol 9:560~567
FUYAN / 2014 / Expression of translationally controlled tumor protein (TCTP) gene of Dirofilaria immitis guided by transcriptomic screening / The Korean Journal of Parasitology 52(1):21~26
Rossi MI / 2010 / Detection of Wolbachia DNA in blood from dogs infected with Dirofilaria immitis / Exp Parasitol 126:270~272
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