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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제22권 제8호
- 발행연도
- 2012.1
- 수록면
- 1,092 - 1,100 (9page)
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초록· 키워드
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Ten Bacillus strains with antimicrobial activities were isolated from Cheonggukjang produced at different parts in Korea. They all inhibited Listeria monocytogenes ATCC 19111 and nine inhibited Bacillus cereus ATCC 14579.
Four isolates (W42, H27, SKE 12, and K21) showing strong inhibiting activities were identified as B. subtilis.
B. subtilis W42 was the most inhibiting strain. The antimicrobial activity of culture supernatant from B.
subtilis W42 was destroyed completely by proteinase K treatment, indicating that a bacteriocin was the responsible agent. The bacteriocin, Bac W42, was most stable at pH 7and stable between pH 3-6 and 8-9. Bac W42 was stable up to 80oC. BHI (brain heart infusion) and TSB (tryptic soy broth) were the best media for the activity (320 AU/ml)followed by LB (160 AU/ml). Bac W42 was partially purified by column chromatographies. The specific activity was increased from 1,151.2 AU/ml to 9,043.5 AU/ml and the final yield was 26.3%. Bac W42 was 5.4 kDa in size as determined by SDS-PAGE. Bac W42 showed bactericidal activity against L. monocytogenes ATCC 19111.
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참고문헌 (22)
참고문헌 신청
Babasaki, K. / 1985 / Subtilosin A, a new antibiotic peptide produced by Bacillus subtilis 168: Isolation, structural analysis, and biogenesis / J. Biochem 98 : 585 ~ 603
Bizani, D. / 2002 / Characterization of a bacteriocin produced by a newly isolated Bacillus sp. strain 8 A / J. Appl. Microbiol 93 : 512 ~ 519
Bradford, M. M. / 1976 / Rapid and sensitive methods for the quantification of microgram quantities of protein utilizing the principle of protein -dye binding / Anal. Biochem
Chehimi, S. / 2007 / Purification and partial amino acid sequence of thuricin S, a new anti-Listeria bacteriocin from Bacillus thuringiensis / Can. J. Microbiol 53 : 284 ~ 29
Cherif, A. / 2001 / Thuricin 7: A novel bacteriocin produced by Bacillus thuringiensis BMG1.7, a new strain isolated from soil / Lett. Appl. Microbiol 32 : 243 ~ 247
Bizani, D. / 2002 / Characterization of a bacteriocin produced by a newly isolated Bacillus sp. strain 8 A / J. Appl. Microbiol 93 : 512 ~ 519
Bradford, M. M. / 1976 / Rapid and sensitive methods for the quantification of microgram quantities of protein utilizing the principle of protein -dye binding / Anal. Biochem
Chehimi, S. / 2007 / Purification and partial amino acid sequence of thuricin S, a new anti-Listeria bacteriocin from Bacillus thuringiensis / Can. J. Microbiol 53 : 284 ~ 29
Cherif, A. / 2001 / Thuricin 7: A novel bacteriocin produced by Bacillus thuringiensis BMG1.7, a new strain isolated from soil / Lett. Appl. Microbiol 32 : 243 ~ 247
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