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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제28권 제4호
- 발행연도
- 2018.1
- 수록면
- 645 - 651 (7page)
이용수
초록· 키워드
오류제보하기
The carcinogenicity of chemicals in the environment is a major concern. Recently, numerous studies have attempted to develop methods for predicting carcinogenicity, including rodent and cell-based approaches. However, rodent carcinogenicity tests for evaluating the carcinogenic potential of a chemical to humans are time-consuming and costly. This study focused on the development of an alternative method for predicting carcinogenicity using quantitative PCR (qPCR) and colon cancer stem cells. A toxicogenomic method, mRNA profiling, is useful for predicting carcinogenicity. Using microarray analysis, we optimized 16 predictive gene sets from five carcinogens (azoxymethane, 3,2’-dimethyl-4-aminobiphenyl, N-ethyl-n-nitrosourea, metronidazole, 4-(n-methyl-n-nitrosamino)-1-(3-pyridyl)-1-butanone) used to treat colon cancer stem cell samples. The 16 genes were evaluated by qPCR using 23 positive and negative carcinogens in colon cancer stem cells. Among them, six genes could differentiate between positive and negative carcinogens with a p-value of ≤0.05. Our qPCR-based prediction system for colon carcinogenesis using colon cancer stem cells is cost- and time-efficient. Thus, this qPCR-based prediction system is an alternative to in vivo carcinogenicity screening assays.
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등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Siegel RL / 2016 / Cancer statistics, 2016 / CA Cancer J. Clin. 66:7~30
Kakarala M / 2008 / Implications of the cancer stem-cell hypothesis for breast cancer prevention and therapy / J. Clin. Oncol. 26:2813~2820
Gusenleitner D / 2014 / Genomic models of short-term exposure accurately predict long-term chemical carcinogenicity and identify putative mechanisms of action / PLoS One 9:e102579
Hartung T / 2009 / Toxicology for the twenty-first century / Nature 460:208~212
Knight A / 2006 / Animal carcinogenicity studies: implications for the REACH system / Altern. Lab. Anim. 34(Suppl 1):139~147
Kakarala M / 2008 / Implications of the cancer stem-cell hypothesis for breast cancer prevention and therapy / J. Clin. Oncol. 26:2813~2820
Gusenleitner D / 2014 / Genomic models of short-term exposure accurately predict long-term chemical carcinogenicity and identify putative mechanisms of action / PLoS One 9:e102579
Hartung T / 2009 / Toxicology for the twenty-first century / Nature 460:208~212
Knight A / 2006 / Animal carcinogenicity studies: implications for the REACH system / Altern. Lab. Anim. 34(Suppl 1):139~147
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