지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제22권 제6호
- 발행연도
- 2012.1
- 수록면
- 866 - 871 (6page)
이용수
초록· 키워드
오류제보하기
A novel cholesterol-based cationic lipid containing a tri-2-hydroxyethylamine head group and ether linker (Chol-THEA) was synthesized and examined as a potent gene delivery vehicle. In the preparation of cationic liposome,the addition of DOPE as helper lipid significantly increased the transfection efficiency. To find the optimum transfection efficiency, we screened various weight ratios of DOPE and liposome/DNA (N/P). The best transfection efficiency was found at the Chol-THEA:DOPE weight ratio of 1:1 and N/P weight ratio of 10~15. Most of the plasmid DNA was retarded by this liposome at the optimum N/P weight ratio of 10. The transfection efficiency of Chol-THEA liposome was compared with DOTAP,Lipofectamine, and DMRIE-C using the luciferase assay and GFP expression. Chol-THEA liposome with low toxicity had better or similar potency of gene delivery compared with commercial liposomes in COS-7, Huh-7,and MCF-7 cells. Therefore, Chol-THEA could be a useful non-viral vector for gene delivery.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Al-Jamal, W. T. / 2007 / Construction of nanoscale multicompartment liposomes for combinatory drug delivery / Int. J. Pharm 331 : 182 ~ 185
Anwer, K. / 2010 / Phase-I clinical trial of IL-12 plasmid/lipopolymer complexes for the treatment of recurrent ovarian cancer / Gene Ther 17 : 360 ~ 369
Bajaj, A. / 2007 / Synthesis and gene transfer activities of novel serum compatible cholesterol-based gemini lipids possessing oxyethylene-type spacers / Bioconjug. Chem 18 :
Farhood, H. / 1995 / The role of dioleoyl phosphatidylethanolamine in cationic liposome mediated gene transfer / Biochim. Biophys. Acta 1235 : 289 ~ 295
Felgner, J. H. / 1994 / Enhanced gene delivery and mechanism studies with a novel series of cationic lipid formulations / J. Biol. Chem 269 : 2550 ~ 2561
Anwer, K. / 2010 / Phase-I clinical trial of IL-12 plasmid/lipopolymer complexes for the treatment of recurrent ovarian cancer / Gene Ther 17 : 360 ~ 369
Bajaj, A. / 2007 / Synthesis and gene transfer activities of novel serum compatible cholesterol-based gemini lipids possessing oxyethylene-type spacers / Bioconjug. Chem 18 :
Farhood, H. / 1995 / The role of dioleoyl phosphatidylethanolamine in cationic liposome mediated gene transfer / Biochim. Biophys. Acta 1235 : 289 ~ 295
Felgner, J. H. / 1994 / Enhanced gene delivery and mechanism studies with a novel series of cationic lipid formulations / J. Biol. Chem 269 : 2550 ~ 2561
이 논문의 저자 정보
최근 본 자료
댓글(0)
0