지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Soybean proteins are widely used for human and animal feeds worldwide. The use of soybean protein has been expanded in the food industry due to their excellent nutritional benefits. But, antinutritional and allergenic factors are present in the raw mature soybean. P34 protein, referred as Gly m Bd 30K, has been identified as a predominant immunodominant allergen. The objective of this research is to identify the genetic mode of P34 protein for the improvement of soybean cultivar with a very low level of P34 protein. Two F_2 populations were developed from the cross of “Pungsannamulkong” x PI567476and “Gaechuck2ho” x PI567476 (very low level of P34 protein). Relative amount of P34 protein was observed by Western blot analysis. The observed data for the progeny of “Pungsannamulkong” and PI567476 were 133 seeds with normal content of P34protein and 35 seeds with very low level of P34 protein (χ^2=1.157, P=0.20-0.30). For the progeny of “Gaechuck#1” and PI567476, the observed data were 177 seeds with normal content of P34 protein and 73 seeds with very low level of P34 protein (χ^2=2.353, P=0.10-0.20). From pooled data, observed data were 310 seeds with normal content of P34 protein and 108 seeds with very low level of P34 protein (χ^2=0.156, P=0.50-0.70). The segregation ratio (3:1) and the Chi-square value obtained from the two populations suggested that P34 protein in mature soybean seed is controlled by a single major gene. Single gene inheritance of P34 protein was confirmed in 32 F_2 derived lines in F_3 seeds, which were germinated from the low level of P34protein obtained from the cross of “Pungsannamulkong” and PI567476. These results may provide valuable information to breed for new soybean line with low level of P34 protein and identification of molecular markers linked to P34 locus.
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참고문헌 (17)
참고문헌 신청
Davies, C.S. / 1986 / Genetic analysis of a null-allele for lipoxygenase-2 in soybean / Crop Sci 26 : 460 ~ 462
Helm, R.M. / 2000 / Mutational analysis of the IgE-binding epitopes of P34/Gly m Bd 30K / J. Allergy Clin Immuno 105 : 378 ~ 384
Herman, E.M. / 2003 / Genetic modification removes an immunodominant allergen from soybean / Plant Physiology 132 : 36 ~ 43
Herman,E / 2005 / Soybean allergenicity and suppression of the immunodominant allergen / Crop Sci 45 : 462 ~ 467
Hildebrand, D.F. / 1981 / Soybeans lacking lipoxygenase / J. Am. oil Chem. Soc 58 : 583 ~ 586
Helm, R.M. / 2000 / Mutational analysis of the IgE-binding epitopes of P34/Gly m Bd 30K / J. Allergy Clin Immuno 105 : 378 ~ 384
Herman, E.M. / 2003 / Genetic modification removes an immunodominant allergen from soybean / Plant Physiology 132 : 36 ~ 43
Herman,E / 2005 / Soybean allergenicity and suppression of the immunodominant allergen / Crop Sci 45 : 462 ~ 467
Hildebrand, D.F. / 1981 / Soybeans lacking lipoxygenase / J. Am. oil Chem. Soc 58 : 583 ~ 586
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