지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Objectives: To examine the effect of extracts of Korean native Cimicifuge species on cell proliferation in vascular smooth muscle cells (VSMC).
Methods: VSMC were isolated from rat aorta. Cell proliferation was assessed by measure of bromodeoxyuridine incorporation into the cells. Differences in Reactive oxygen species (ROS) levels were examined after exposure to the extracts of Korean native Cimicifuge species using the detection reagents dichlorofluorecin diacetate. The rhizomes/roots were air-dried and milled with a commercial food mixer. Milled rhizomes/roots of each Cimicifuga species were separately extracted by 80% ethanol, absolute methanol, and 40% 2-propanol using homogenizer and evaporated under reduced pressure at low temperatures. Effects of extracts dissolved in phosphate-buffered saline (0.3 mg/mL) were examined.
Results: Ethanolic, methanolic or propanolic extracts of 4 Korean native Cimicifuge species (Cimicifuga [C] davurica, C. japonica, C. heracleifolia var. bifida Nakai, C. simplex) were screened. The addition of extracts of each Korean native Cimicifuge species to cells in the presence of 10% fetal bovine serum (FBS) potently inhibited cell proliferation. Significant decrease of 23%-30% was observed. Vitamin E, a potent antioxidant, inhibited 10% FBS-stimulated cell proliferation of VSMC. We also demonstrated that extracts of each Korean native Cimicifuge species decreased intracellular ROS generation induced with 10% FBS. The effect of Korean native Cimicifuge species was not species-specific and solvent-specific.
Conclusion: TExtracts of Korean native Cimicifuge species inhibit VSMC proliferation via inhibition of intracellular ROS. These findings suggest that Cimicifuge species used for reducing menopause symptoms might be cardioprotective in women.
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참고문헌 (25)
참고문헌 신청
Anthony MS / 1996 / Soybean isoflavones improve cardiovascular risk factors without affecting the reproductive system of peripubertal rhesus monkeys / J Nutr 126 : 43 ~ 50
Barrett-Connor E / 1998 / Hormone replacement therapy, heart disease, and other considerations / Annu Rev Public Health 19 : 55 ~ 72
Catapano AL / 2000 / Low density lipoprotein oxidation, antioxidants, and atherosclerosis / Curr Opin Cardiol 15 : 355 ~ 363
Chung DJ / 2006 / Black cohosh and St. John’s Wort (GYNO-Puls®) for climacteric symptoms: a double-blind, randomized, placebo-controlled, multicenter trial / J Korean Soc
Foster S / 1999 / Black cohosh: a literature review / HerbalGram 45 : 35 ~ 50
Barrett-Connor E / 1998 / Hormone replacement therapy, heart disease, and other considerations / Annu Rev Public Health 19 : 55 ~ 72
Catapano AL / 2000 / Low density lipoprotein oxidation, antioxidants, and atherosclerosis / Curr Opin Cardiol 15 : 355 ~ 363
Chung DJ / 2006 / Black cohosh and St. John’s Wort (GYNO-Puls®) for climacteric symptoms: a double-blind, randomized, placebo-controlled, multicenter trial / J Korean Soc
Foster S / 1999 / Black cohosh: a literature review / HerbalGram 45 : 35 ~ 50
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