지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이용수
초록· 키워드
오류제보하기
Background: The light-emitting diode (LED) curing light used is presumed to be safe. However, the scientific basis for this is unclear, and the safety of LED curing light is still controversial. The purpose of this study was to investigate the effect of LED curing light irradiation according to the conditions applied for the polymerization of composite resins in dental clinic on the cell viability and inflammatory response in Raw264.7 macrophages and to confirm the stability of LED curing light. Methods: Cell viability and cell morphology of Raw264.7 macrophages treated with 100 ng/ml of lipopolysaccharide (LPS) or/and LED curing light with a wavelength of 440∼490 nm for 20 seconds were confirmed by methylthiazolydiphenyl-tetrazolium bromide assay and microscopic observation. The production of nitric oxide (NO) and prostaglandin E2 (PGE2) was confirmed by NO assay and PGE2 enzyme-linked immunosorbent assay kit. Expression of interleukin (IL)-1 and tumor necrosis factor (TNF)- in total RNA and protein was confirmed by reverse transcription polymerase chain reaction and Western blot analysis. Results: The LED curing light did not affect the viability and morphology of normal Raw264.7 cells but affected the cell viability and induced cytotoxicity in the inflammation-induced Raw264.7 cells by LPS. The irradiation of the LED curing light did not progress to the inflammatory state in the inflammation-induced Raw264.7 macrophage. However, LED curing light irradiation in normal Raw264.7 cells induced an increase in NO and PGE2 production and mRNA and protein expression of IL-1 and TNF-, indicating that it is possible to induce the inflammatory state. Conclusion: The irradiation of LED curing light in RAW264.7 macrophage may induce an excessive inflammatory reaction and damage oral tissues. Therefore, it is necessary to limit the long-term irradiation which is inappropriate when applying LED curing light in a dental clinic.
목차
등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
J. C. WATAHA / 2008 / Response of THP-1 monocytes to blue light from dental curing lights / Journal of Oral Rehabilitation 35(2):105~110
J Wataha / 2003 / In vitro biological response to core and flowable dental restorative materials / Dental Materials 19(1):25~31
Nakamura S / 1994 / Candela‐class highbrightness InGaN/AlGaN double‐heterostructure blue‐lightemitting diodes / Appl Phys Lett 64:1687~1689
Cristina Kurachi / 2001 / Hardness evaluation of a dental composite polymerized with experimental LED-based devices / Dental Materials 17(4):309~315
Ergun G / 2010 / The effect of light curing units and modes on cytotoxicity of resin-core systems / Med Oral Patol Oral Cir Bucal 15:e962~e968
J Wataha / 2003 / In vitro biological response to core and flowable dental restorative materials / Dental Materials 19(1):25~31
Nakamura S / 1994 / Candela‐class highbrightness InGaN/AlGaN double‐heterostructure blue‐lightemitting diodes / Appl Phys Lett 64:1687~1689
Cristina Kurachi / 2001 / Hardness evaluation of a dental composite polymerized with experimental LED-based devices / Dental Materials 17(4):309~315
Ergun G / 2010 / The effect of light curing units and modes on cytotoxicity of resin-core systems / Med Oral Patol Oral Cir Bucal 15:e962~e968
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
In vitro investigation of the antibacterial and anti-inflammatory effects of LED irradiation
Journal of Periodontal & Implant Science
2023 .04
-
Errors in light-emitting diodes positioning when curing bulk fill and incremental composites: impact on properties after aging
Restorative Dentistry and Endodontics
2021 .11
-
Effect of tack cure time on polymerization shrinkage of dual-cure resin cement
International Journal of Oral Biology
2021 .12
-
다양한 중합 방법으로 제작한 간접 수복물용 복합레진의 굴곡 강도
대한치과기공학회지
2018 .01
-
LPS유도 대식세포에서 Kaempferol-7-O-β-D-glucoside의 NO, PGE₂ 및 염증성 사이토카인 생성 저해 효과
생약학회지(Korean Journal of Pharmacognosy)
2016 .12
-
Anti-inflammatory Effects of Complex Extract including Eucommia ulmoides in LPS-induced RAW 264.7 Cells
대한침구의학회지
2019 .01
-
Influence of irradiation distance on the mechanical performances of resin composites polymerized with high-irradiance light curing units
생체재료학회지
2022 .06
-
Comparison of Surface Microhardness of the Flowable Bulk-Fill Resin and the Packable Bulk-Fill Resin according to Light Curing Time and Distance
치위생과학회지
2023 .06
-
Comparison of polymerization shrinkage of dual-cure core build-up resin according to shade and curing mode
Oral Biology Research
2019 .01
-
광조사기의 최신지견
대한통합치과학회지
2019 .01
-
Application of a Non-Mixture Cure Rate Model for Analyzing Survival of Patients with Breast Cancer
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
Anti-inflammatory Effects of Ethanol Extract of Chinese Medicinal Plants in Yanjin on LPS-stimulated RAW 264.7 Macrophages
대한본초학회지(본초분과학회지)
2018 .01
-
LPS로 유발한 RAW264.7 세포 염증반응에 대한 가감통순산(加減通順散)의 억제 효과
한방재활의학과학회지
2018 .01
-
Anti-inflammatory Effect of Mangosteen (Garcinia mangostana L.) Peel Extract and its Compounds in LPS-induced RAW264.7 Cells
Natural Product Sciences
2016 .09
-
Biological Effects of Light-Emitting Diodes Curing Unit on MDPC-23 Cells and Lipopolysaccharide Stimulated MDPC-23 Cells
치위생과학회지
2019 .01
-
Fitting Cure Rate Model to Breast Cancer Data of Cancer Research Center
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
선학초(仙鶴草)추출물의 대식세포에서의 LPS-유도 염증반응에 대한 효능 연구
대한본초학회지(본초분과학회지)
2016 .01
-
꽃송이버섯 추출물이 RAW 264.7 세포에서 TNF-α, iNOS, IL-1β 유전자 발현에 미치는 영향
대한통합의학회지
2021 .01
-
Anti-inflammatory Effects of Ethanol Extract of Various Korean Compositae Herbs in LPS-induced RAW 264.7 Macrophages
대한본초학회지(본초분과학회지)
2017 .01
-
紫草 열수 추출물의 RAW 264.7 세포에서 IL-1β, TNF-α, iNOS 유전자 발현에 미치는 영향 연구
동의생리병리학회지
2017 .08
이 논문의 저자 정보
최근 본 자료
댓글(0)
0