지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.10
- 수록면
- 666 - 675 (10page)
이용수
초록· 키워드
오류제보하기
Background: Korean Red Ginseng (KRG) has been widely used as an herbal medicine to normalize and strengthen body functions. Although many researchers have focused on the biological effects of KRG, more studies on the action mechanism of red ginseng are still needed. Previously, we investigated the proteomic changes of the rat spleen while searching for molecular signatures and the action mechanism of KRG. The proteomic analysis revealed that differentially expressed proteins (DEPs) were involved in the increased immune response and phagocytosis. The aim of this study was to evaluate the biological activities of KRG, especially the immune-enhancing response of KRG.
Methods: Rats were divided into 4 groups: 0 (control group), 500, 1000, and 2000 mg/kg administration of KRG powder for 6 weeks, respectively. Isobaric tags for relative and absolute quantitation was performed with Q-Exactive LC-MS/MS to compare associated proteins between the groups. The putative DEPs were identified by a current UniProt rat protein database search and by the Gene Ontology annotations.
Results: The DEPs appear to increase the innate and acquired immunity as well as immune cell movement. These results suggest that KRG can stimulate immune responses. This analysis refined our targets of interest to include the potential functions of KRG. Furthermore, we validated the potential molecular targets of the functions, representatively LCN2, CRAMP, and HLA-DQB1, by Western blotting.
Conclusion: These results may provide molecular signature candidates to elucidate the mechanisms of the immune response by KRG. Here, we demonstrate a strategy of tissue proteomics for the discovery of the molecular function of KRG.
Methods: Rats were divided into 4 groups: 0 (control group), 500, 1000, and 2000 mg/kg administration of KRG powder for 6 weeks, respectively. Isobaric tags for relative and absolute quantitation was performed with Q-Exactive LC-MS/MS to compare associated proteins between the groups. The putative DEPs were identified by a current UniProt rat protein database search and by the Gene Ontology annotations.
Results: The DEPs appear to increase the innate and acquired immunity as well as immune cell movement. These results suggest that KRG can stimulate immune responses. This analysis refined our targets of interest to include the potential functions of KRG. Furthermore, we validated the potential molecular targets of the functions, representatively LCN2, CRAMP, and HLA-DQB1, by Western blotting.
Conclusion: These results may provide molecular signature candidates to elucidate the mechanisms of the immune response by KRG. Here, we demonstrate a strategy of tissue proteomics for the discovery of the molecular function of KRG.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results
4. Discussion
References
참고문헌 (48)
참고문헌 신청
양하나 / 2016 / Effect of Korean Red Ginseng treatment on the gene expression profile of diabetic rat retina / Journal of Ginseng Research 40(1):1~8
김신정 / 2016 / Changes in the contents of prosapogenin in Red ginseng (Panax ginseng) depending on the extracting conditions / Journal of Ginseng Research 40(1):86~89
Xie YY / 2012 / Steaming-induced chemical transformations and holistic quality assessment of red ginseng derived from Panax ginseng by means of HPLC-ESI-MS/MS(n)-based multicomponent quantification fingerprint / J Agric Food Chem 60:8213~8224
Provino R / 2010 / The role of adaptogens in stress management / Australian Journal of Medical Herbalism 22:41
서성옥 / 2004 / 진행성 위암 환자에서 수술 후 홍삼엑기스에 의한 면역 조절자 역할에 관한 전향적 연구 / Journal of Ginseng Research 28(2):104~110
김신정 / 2016 / Changes in the contents of prosapogenin in Red ginseng (Panax ginseng) depending on the extracting conditions / Journal of Ginseng Research 40(1):86~89
Xie YY / 2012 / Steaming-induced chemical transformations and holistic quality assessment of red ginseng derived from Panax ginseng by means of HPLC-ESI-MS/MS(n)-based multicomponent quantification fingerprint / J Agric Food Chem 60:8213~8224
Provino R / 2010 / The role of adaptogens in stress management / Australian Journal of Medical Herbalism 22:41
서성옥 / 2004 / 진행성 위암 환자에서 수술 후 홍삼엑기스에 의한 면역 조절자 역할에 관한 전향적 연구 / Journal of Ginseng Research 28(2):104~110
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UCI(KEPA) : I410-ECN-0101-2020-524-000504768