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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 591 - 601 (11page)
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Controlling root-knot nematodes (Meloidogyne spp.) using RNA interference (RNAi)-based molecular strategy is currently gaining consideration as a safer alternative to the use of chemical nematicides. However, identifying target genes whose knockdown in the parasites can effectively protect host plants is critical to success of this strategy. In this study, we trans-formed tomato plants to express hairpin RNA of Meloidogyne incognita-specific sequence (MiPA) of PolA1, an essential single-copy nuclear gene encoding the largest subunit of RNA polymerase I enzyme. We then evaluated nematode resist-ance in T1 transgenic lines for efficacy of host plant-mediated silencing of PolA1 gene in invading nematodes. We observed a significant reduction in nematode egg masses per plant root, eggs per egg mass and overall parasite multiplication in the transgenic plants compared to the wild type. Transgenic plants also had a reduced number of nematode galls per plant root than wild type but not significant. Although agronomic traits evaluated at the early growth stage were comparable in trans-genics and wild types of tomato plants, production of MiPA-specific siRNAs in transgenic plants as well as the significant reduction of nematode PolA1 gene expression in feeding nematodes isolated from roots of transgenic plants provided evi-dence of host-induced gene silencing. Put together, our results are an indication that silencing of PolA1 gene is useful for reducing propagation of root-knot nematodes in tomato hosts. Its application to other crops may be possible and useful in improving agricultural productivity.
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