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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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초록· 키워드
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Chlorella gloriosa (Chlorellaceae, Trebouxiophyceae) was isolated from seawater off the coast of the Dokdo Islands in Korea. An axenic culture was established using the streak-plate method on f/2 agar media supplemented with antibiotics, allowing identification of the isolate by morphological, molecular, and physiological analyses. The morphological characteristics observed by light and electron microscopy revealed typical morphologies of C. gloriosa species. The molecular phylogenetic inference drawn from the small-subunit 18S rRNA sequence verified that the microalgal strain belongs to C. gloriosa. Additionally, gas chromatography-mass spectrometry analysis showed that the isolate was rich in nutritionally important omega-3 and -6 polyunsaturated fatty acids and high-performance liquid chromatography analysis revealed that the high-value antioxidants lutein and violaxanthin were biosynthesized as accessory pigments by this microalga, with arabinose, galactose, and glucose as the major monosaccharides. Therefore, in this study, a Korean marine C. gloriosa species was discovered, characterized, and described, and subsequently added to the national culture collection.
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참고문헌 (40)
참고문헌 신청
Ausich RL / 1997 / Commercial opportunities for carotenoid production by biotechnology / Pure Appl. Chem 69:2169~2173
배진희 / 2011 / Selection of Suitable Species of Chlorella, Nannochloris, and Nannochloropsis in High- and Low-Temperature Seasons for Mass Culture of the Rotifer Brachionus plicatilis / Fisheries and Aquatic Sciences 14(4):323~332
Beijerinck MW / 1890 / Culturversuche mit Zoochlorellen, Lichenengonidien und anderen niederen Algen / Bot. Zeitung 47:725~785
Bock C / 2011 / Taxonomic reassessment of the genus Chlorella(Trebouxiophyceae)using molecular signatures(barcodes), including description of seven new species / Fottea 11:293~312
Breuer G / 2013 / Analysis of fatty acid content and composition in microalgae / J. Vis. Exp 80:e50628
배진희 / 2011 / Selection of Suitable Species of Chlorella, Nannochloris, and Nannochloropsis in High- and Low-Temperature Seasons for Mass Culture of the Rotifer Brachionus plicatilis / Fisheries and Aquatic Sciences 14(4):323~332
Beijerinck MW / 1890 / Culturversuche mit Zoochlorellen, Lichenengonidien und anderen niederen Algen / Bot. Zeitung 47:725~785
Bock C / 2011 / Taxonomic reassessment of the genus Chlorella(Trebouxiophyceae)using molecular signatures(barcodes), including description of seven new species / Fottea 11:293~312
Breuer G / 2013 / Analysis of fatty acid content and composition in microalgae / J. Vis. Exp 80:e50628
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