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논문 기본 정보

자료유형
학술저널
저자정보
Kim, Sang-Hwan (Institute of Genetic Engineering, Hankyong National University) Kim, Kyong-Lae (Institute of Genetic Engineering, Hankyong National University) Lee, Ji-Hye (Major in the Animal Biotechnology, Graduate School of Future Convergence Technology, Hankyong National University) Shin, Da-Hye (Department of Animal Life and Environment science, Graduate School of Future Convergence Technology, Hankyong National University) Jung, Na-Hyeon (Department of Animal Life and Environment science, Graduate School of Future Convergence Technology, Hankyong National University) Lee, Ho-Jun (Institute of Genetic Engineering, Hankyong National University) Yoon, Jong-Taek (Institute of Genetic Engineering, Hankyong National University)
저널정보
한국동물번식학회 한국수정란이식학회지 한국수정란이식학회지 제33권 제2호
발행연도
2018.1
수록면
61 - 68 (8page)

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The major focus of this study is to analyze the expression of bovine MMPs and to monitor their activity during the estrus cycle and pregnancy. During pregnancy, MMP-2 expression was detectable around 30 days but became insignificant by 60 days, then started to increase again around 90 days and reached the maximum at 250 days. The activity of MMP-2 protein changed in accordance with its expression level. As expected, the level of TIMP-2 exhibited a reverse pattern. About MMP-9, high level expression was observed as early as 30 days and gradually increase until 90 days. Then started to decrease after 250 days. Again, the sites of MMP-9 expression were similar to those of MMP-2. On the other hand, expression of TIMP-3 remained low until 90 days but showed a small and temporal increase around 250 days. In summary, expression of different MMPs were differentially regulated during estrus cycle and pregnancy. While the expression of MMP-2 was high in estrus cycle, MMP-9 slowly takes over with the progression of pregnancy. These results indicated that the luteal tissue perform distinct functions during pregnancy and estrus. Perhaps the activity of MMP-2 is required for the structural remodeling of luteum, resulting the suppression of P4 inflow from blood. On the other hand, steady maintenance of MMP-9 throughout luteal development is important for the activation of cell proliferation, maturation and angiogenesis.

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