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- 자료유형
- 학술저널
- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제30권 제11호
- 발행연도
- 2020.1
- 수록면
- 1,777 - 1,784 (8page)
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To increase the availability of microalgae as producers of valuable compounds, it is necessary to develop novel systems for gene expression regulation. Among the diverse expression systems available in microalgae, none are designed to induce expression by low temperature. In this study, we explored a cold-inducible system using the antifreeze protein (AFP) promoter from a polar diatom, Chaetoceros neogracile. A vector containing the CnAFP promoter (pCnAFP) was generated to regulate nuclear gene expression, and reporter genes (Gaussia luciferase (GLuc) and mVenus fluorescent protein (mVenus)) were successfully expressed in the model microalga, Chlamydomonas reinhardtii. In particular, under the control of pCnAFP, the expression of these genes was increased at low temperature, unlike pAR1, a promoter that is widely used for gene expression in C. reinhardtii. Promoter truncation assays showed that cold inducibility was still present even when pCnAFP was shortened to 600 bp, indicating the presence of a low-temperature response element between ?600 and ?477 bp. Our results show the availability of new heterologous gene expression systems with cold-inducible promoters and the possibility to find novel low-temperature response factors in microalgae. Through further improvement, this cold-inducible promoter could be used to develop more efficient expression tools.
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참고문헌 (46)
참고문헌 신청
Potvin G / 2010 / Strategies for high-level recombinant protein expression in transgenic microalgae : a review / Biotechnol. Adv 28:910~918
Bajhaiya AK / 2017 / Transcriptional engineering of microalgae : prospects for high-value chemicals / Trends Biotechnol 35:95~99
Pulz O / 2004 / Valuable products from biotechnology of microalgae / Appl. Microbiol. Biotechnol 65:635~648
Cordero BF / 2011 / Enhancement of lutein production in Chlorella sorokiniana(Chorophyta)by improvement of culture conditions and random mutagenesis / Mar. Drugs 9:1607~1624
Kim M / 2017 / Development of a Dunaliella tertiolecta strain with increased zeaxanthin content using random mutagenesis / Mar. Drugs 15:189
Bajhaiya AK / 2017 / Transcriptional engineering of microalgae : prospects for high-value chemicals / Trends Biotechnol 35:95~99
Pulz O / 2004 / Valuable products from biotechnology of microalgae / Appl. Microbiol. Biotechnol 65:635~648
Cordero BF / 2011 / Enhancement of lutein production in Chlorella sorokiniana(Chorophyta)by improvement of culture conditions and random mutagenesis / Mar. Drugs 9:1607~1624
Kim M / 2017 / Development of a Dunaliella tertiolecta strain with increased zeaxanthin content using random mutagenesis / Mar. Drugs 15:189
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