Circular RNA hsa_circ_0005556 Accelerates Gastric Cancer Progression by Sponging miR-4270 to Increase MMP19 Expression

Duo Shen; Hongyu Zhao; Peng Zeng; Jinyun Song; Yiqiong Yang; Xuefeng Gu; Qinghua Ji; Wei Zhao

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자료유형: 학술저널

저자정보: Duo Shen (Southeast University) Hongyu Zhao (Nanjing University of Chinese Medicine) Peng Zeng (Southeast University) Jinyun Song (Nanjing University of Chinese Medicine) Yiqiong Yang (Southeast University) Xuefeng Gu (Southeast University) Qinghua Ji (Southeast University) Wei Zhao (Southeast University)

저널정보: 대한위암학회 Journal of Gastric Cancer Journal of Gastric Cancer 제20권 제3호

발행연도: 2020.1

수록면: 300 - 312 (13page)

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Purpose: Circular RNAs (circRNAs) are a new class of RNA molecules whose function is largely unknown. There is a growing evidence that circRNAs play an important regulatory role in the progression of a variety of human cancers. However, the exact roles and the mechanisms of circRNAs in gastric cancer are not clear. In this study, we aimed to elucidate the mechanism of hsa_circ_0005556. Materials and Methods: Real-time quantitative polymerase chain reaction was used to detect the expression of hsa_circ_0005556, miR-4270, and matrix metalloproteinase-19 (MMP19) in gastric cancer tissues and cell lines. The expression of hsa_circ_0005556 in gastric cancer cells was silenced by lentivirus, and cell proliferation, invasion, migration, and tumorigenesis in nude mice were assessed to evaluate the function of hsa_circ_0005556 in gastric cancer. Results: The expression of hsa_circ_0005556 in gastric cancer tissues and gastric cancer cell lines was higher compared to normal controls. In vitro, the downregulation of hsa_circ_0005556 significantly inhibited proliferation, migration, and invasion of gastric cancer cells. In vivo, the downregulation of hsa_circ_0005556 suppressed tumor growth in nude mice. Conclusions: Our study shows that the hsa_circ_0005556/miR-4270/MMP19 axis is involved in proliferation, migration, and invasion of gastric cancer cells through the competing endogenous RNA (ceRNA) mechanism.

#CircRNAs #MMP19 #miRNA sponge #Gastric cancer

참고문헌 (28)

참고문헌 신청

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