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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.1
- 수록면
- 133 - 140 (8page)
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초록· 키워드
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Background Massively parallel sequencing (MPS) technology has recently been introduced in research, clinical diagnostics, and forensics. MPS enables determination of the genotypes of multiple short tandem repeat (STR) markers and to determine nucleotide sequence variations, additionally. Objective To improve STR analysis and a paternity index, a new, smaller-sized STR panel was designed that includes the SE33 locus. Methods This study performed MPS using an STR panel including the SE33 marker in 101 Koreans. The concordance study was conducted by comparing the data obtained from the MPS assay with the results of a capillary electrophoresis (CE)-based method. Results In this study, an in-house MPS panel is designed that incorporates the 20 Combined DNA Index System (CODIS) loci and the Penta D, Penta E, and SE33 markers for enhanced discriminatory ability. The data obtained via MPS analysis were compared with CE data to confirm concordance. Fifty previously unreported alleles were detected through the MPS analysis. Three new SNP variations in the flanking region were also identified. Statistical analysis demonstrated that the SE33 marker was most effectively determined the match probability (PM) and typical paternity index (TPI). In the sensitivity study, concentrations as low as 80 pg could be used to obtain full and concordant profiles. Conclusions We designed a new, smaller-sized STR panel that includes the SE33 locus to improve STR analysis and the paternity index. Various new alleles were identified in SE33, indicating a high degree of polymorphism. The panel is expected to provide valid data for discrimination of unidentified bodies.
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참고문헌 (28)
참고문헌 신청
Noora R. Al-Snan / 2019 / Population genetic data of the 21 autosomal STRs included in GlobalFiler kit of a population sample from the Kingdom of Bahrain / PLOS ONE 14(8):e0220620~14
David Ballard / 2020 / Massive parallel sequencing in forensics: advantages, issues, technicalities, and prospects / International Journal of Legal Medicine 134(4):1291~1303
A. Barbaro / 2011 / Genetic data for the locus SE33 in a Southern Italy population with AmpFlSTR NGM SElect™ PCR amplification kit / Forensic Science International: Genetics Supplement Series 3(1):e101~e102
Pedro A. Barrio / 2019 / Massively parallel sequence data of 31 autosomal STR loci from 496 Spanish individuals revealed concordance with CE-STR technology and enhanced discrimination power / Forensic Science International: Genetics 42:49~55
Eva C Berglund / 2011 / Next-generation sequencing technologies and applications for human genetic history and forensics / Investigative Genetics 2(1)
David Ballard / 2020 / Massive parallel sequencing in forensics: advantages, issues, technicalities, and prospects / International Journal of Legal Medicine 134(4):1291~1303
A. Barbaro / 2011 / Genetic data for the locus SE33 in a Southern Italy population with AmpFlSTR NGM SElect™ PCR amplification kit / Forensic Science International: Genetics Supplement Series 3(1):e101~e102
Pedro A. Barrio / 2019 / Massively parallel sequence data of 31 autosomal STR loci from 496 Spanish individuals revealed concordance with CE-STR technology and enhanced discrimination power / Forensic Science International: Genetics 42:49~55
Eva C Berglund / 2011 / Next-generation sequencing technologies and applications for human genetic history and forensics / Investigative Genetics 2(1)
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