The Integrins Involved in Soybean Agglutinin-Induced Cell Cycle Alterations in IPEC-J2

Li Pan; Yuan Zhao; Zhijie Yuan; Mohammed Hamdy Farouk; Shiyao Zhang; Nan Bao; GuiXin Qin

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자료유형: 학술저널

저자정보: Li Pan (Jilin Agricultural University) Yuan Zhao (Jilin Agricultural University) Zhijie Yuan (Jilin Agricultural University) Mohammed Hamdy Farouk (Jilin Agricultural University) Shiyao Zhang (Jilin Agricultural University) Nan Bao (Jilin Agricultural University) GuiXin Qin (Jilin Agricultural University)

저널정보: 한국분자세포생물학회 Molecules and Cells Molecules and Cells 제40권 제2호

발행연도: 2017.2

수록면: 109 - 116 (8page)

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Soybean agglutinin (SBA) is an anti-nutritional factor of soybean, affecting cell proliferation and inducing cytotoxicity. Integrins are transmembrane receptors, mediating a variety of cell biological processes. This research aims to study the effects of SBA on cell proliferation and cell cycle progression of the intestinal epithelial cell line from piglets (IPEC-J2), to identify the integrin subunits especially expressed in IPEC-J2s, and to analyze the functions of these integrins on IPEC-J2 cell cycle progression and SBA-induced IPEC-J2 cell cycle alteration. The results showed that SBA lowered cell proliferation rate as the cell cycle progression from G0/G1 to S phase (P < 0.05) was inhibited. Moreover, SBA lowered mRNA expression of cell cycle-related gene CDK4, Cyclin E and Cyclin D1 (P < 0.05). We successfully identified integrins ?2, ?3, ?6, ?1, and ?4 in IPEC-J2s. These five subunits were crucial to maintain normal cell proliferation and cell cycle progression in IPEC-J2s. Restrain of either these five subunits by their inhibitors, lowered cell proliferation rate, and arrested the cells at G0/G1 phase of cell cycle (P < 0.05). Further analysis indicated that integrin ?2, ?6, and ?1 were involved in the blocking of G0/G1 phase induced by SBA. In conclusion, these results suggested that SBA lowered the IPEC-J2 cell proliferation rate through the perturbation of cell cycle progression. Furthermore, integrins were important for IPEC-J2 cell cycle progression, and they were involved in the process of SBA-induced cell cycle progression alteration, which provide a basis for further revealing SBA anti-proliferation and anti-nutritional mechanism.

#cell cycle #cell proliferation #functional mechanism #integrin subunits #soybean agglutinin

참고문헌 (36)

참고문헌 신청

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