Nutraceutical emulsion containing valproic acid (NE-VPA): a drug delivery system for reversion of seizures in zebrafish larvae epilepsy model

Daniela Agustina Feas; Daniela Edith Igartúa; María Natalia Calienni; Carolina Soledad Martinez; Marina Pifano; Nadia Silvia Chiaramoni; Silvia del Valle Alonso; María Jimena Prieto

추천

검색

자료유형: 학술저널

저자정보: Daniela Agustina Feas (Universidad Nacional de Quilmes Argentina) Daniela Edith Igartúa (Universidad Nacional de Quilmes Argentina) María Natalia Calienni (Universidad Nacional de Quilmes Argentina) Carolina Soledad Martinez (Universidad Nacional de Quilmes Argentina) Marina Pifano (Universidad Nacional de Quilmes Argentina) Nadia Silvia Chiaramoni (Universidad Nacional de Quilmes Argentina) Silvia del Valle Alonso (Universidad Nacional de Quilmes Argentina) María Jimena Prieto (Universidad Nacional de Quilmes Argentina)

저널정보: 한국약제학회 Journal of Pharmaceutical Investigation Journal of Pharmaceutical Investigation 제47권 제5호

발행연도: 2017.9

수록면: 429 - 437 (9page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Valproic acid (VPA) is an antiepileptic drug, which is currently used in neurodegenerative diseases. However, a high dose is required to obtain a therapeutic effect. Long-chain polyunsaturated fatty acids (PUFAs), such as omega 3 and omega 6, are efficient complements in treatments for neurological diseases. Previous studies have reported that a dietary supplement containing PUFAs together with the administration of antiepileptic drugs significantly reduces the frequency of seizures. Based on this, the main goal of this work was to obtain a complex based on VPA encapsulation in an oil/water (o/w) nutraceutical emulsion (NE) enriched with PUFAs for oral administration. Besides, encapsulation of VPA might reduce its dose and increase its therapeutic effect. In order to study its effect, we used a zebrafish larvae model of induced epileptiform behavior with the proconvulsant drug pentylenetetrazol (PTZ). Results have shown that when 100 μM VPA and fatty acids were combined in the NE (NE-VPA), the epileptiform behavior of PTZ-treated zebrafish larvae decreased significantly. Additionally, morphological changes, hepatotoxicity, lethality and heart rate were studied. Despite the fact that a high dose of VPA exerted a cardiotoxic effect, this was no longer detected after addition of this drug in the NE. This treatment exerted a significant antiepileptic effect and did not result in highly toxic or lethal effects. In order to develop an improved pharmaceutical treatment, and considering that all the components used are FDA approved for consumption, the NE-VPA selected might be easily incorporated into clinical trials.

#Epilepsy #Valproic acid #Emulsion #Oral drug delivery #Toxicity #Zebrafish

참고문헌 (23)

참고문헌 신청

Afrikanova T / 2013 / Validation of the zebrafish pentylenetetrazol seizure model: locomotor versus electrographic responses to antiepileptic drugs / PLoS ONE 8(1):e54166

Balasubramanian D / 2015 / Valproic acid exposure leads to upregulation and increased promoter histone acetylation of sepiapterin reductase in a serotonergic cell line / Neuropharmacology 99:79~88

Bar-Ilan O / 2009 / Toxicity assessments of multisized gold and silver nanoparticles in zebrafish embryos / Small 5(16):1897~1910

Fisher RS / 2005 / Epileptic seizures and epilepsy: definitions proposed by the International League Against Epilepsy (ILAE) and the International Bureau for Epilepsy (IBE) / Epilepsia 46(4):470~472

Gumpricht E / 2014 / Can ω-3 fatty acids and tocotrienolrich vitamin E reduce symptoms of neurodevelopmental disorders? / Nutrition 30(7):733~738

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!