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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제6호
- 발행연도
- 2017.6
- 수록면
- 1,128 - 1,137 (10page)
- DOI
- 10.4014/jmb.1612.12036
이용수
초록· 키워드
오류제보하기
The aim of this study was to explore the accuracy and feasibility of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) in identifying bacteria from environmental sources, as compared with rpoA gene sequencing, and to evaluate the occurrence of bacteria of the genus Enterococcus in wild birds. In addition, a phyloproteomic analysis of certain Enterococcus species with spectral relationships was performed. The enterococci were isolated from 25 species of wild birds in central Europe (Poland). Proteomic (MALDI-TOF MS) and genomic (rpoA gene sequencing) methods were used to identify all the isolates. Using MALDI-TOF MS, all 54 (100%) isolates were identified as Enterococcus spp. Among these, 51 (94.4%) isolates were identified to the species level (log(score) ≥2.0), and three isolates (5.6%) were identified at a level of probable genus identification (log(score) 1.88-1.927). Phylogenetic analysis based on rpoA sequences confirmed that all enterococci had been correctly identified. Enterococcus faecalis was the most prevalent enterococcal species (50%) and Enterococcus faecium (33.3%) the second most frequent species, followed by Enterococcus hirae (9.3%), Enterococcus durans (3.7%), and Enterococcus casseliflavus (3.7%). The phyloproteomic analysis of the spectral profiles of the isolates showed that MALDI-TOF MS is able to differentiate among similar species of the genus Enterococcus.
목차
등록된 정보가 없습니다.
참고문헌 (36)
참고문헌 신청
Dolka B / 2016 / Phenotypic and genotypic characterization of Enterococcus cecorum strains associated with infections in poultry / BMC Vet. Res 12:129
Werner G / 2012 / Improved identification including MALDI-TOF mass spectrometry analysis of group D streptococci from bovine mastitis and subsequent molecular characterization of corresponding Enterococcus faecalis and Enterococcus faecium isolates / Vet. Microbiol 160:162~169
Marrow J / 2009 / Prevalence and antibiotic-resistance characteristics of Enterococcus spp. isolated from free-living and captive raptors in Central Illinois / J. Wildl. Dis 45:302~313
Radhouani H / 2012 / Wild birds as biological indicators of environmental pollution: antimicrobial resistance patterns of Escherichia coli and enterococci isolated from common buzzards (Buteo buteo) / J. Med. Microbiol 61:837~843
Santos T / 2013 / Dissemination of antibiotic resistant Enterococcus spp. and Escherichia coli from wild birds of Azores Archipelago / Anaerobe 24:25~31
Werner G / 2012 / Improved identification including MALDI-TOF mass spectrometry analysis of group D streptococci from bovine mastitis and subsequent molecular characterization of corresponding Enterococcus faecalis and Enterococcus faecium isolates / Vet. Microbiol 160:162~169
Marrow J / 2009 / Prevalence and antibiotic-resistance characteristics of Enterococcus spp. isolated from free-living and captive raptors in Central Illinois / J. Wildl. Dis 45:302~313
Radhouani H / 2012 / Wild birds as biological indicators of environmental pollution: antimicrobial resistance patterns of Escherichia coli and enterococci isolated from common buzzards (Buteo buteo) / J. Med. Microbiol 61:837~843
Santos T / 2013 / Dissemination of antibiotic resistant Enterococcus spp. and Escherichia coli from wild birds of Azores Archipelago / Anaerobe 24:25~31
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