지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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초록· 키워드
오류제보하기
RabA1a is a small GTPase that contain a conserved GTP binding pocket to promote GTP hydrolysis. Fifty-seven isotypesof Rab GTPase have been found in Arabidopsis thaliana, and the twenty-seven RabA proteins are classified into sixsubgroups, from RabA1 to RabA6. RabA members play crucial roles in controlling docking and fusion during vesicletransport, yet their molecular and physiological functions remain unknown. Moreover, no RabA group protein structureshave yet been determined. In this study, in order to understand the structural features and regulatory mechanisms of RabAfrom the crystal structures, an engineered N-terminal His-tagged RabA1a protein was overexpressed and purified. TheRabA1a protein was crystallized with either GDP for an inactive form or GppNHp as a non-hydrolyzable GTP analog foran active form. X-ray diffraction data from crystals of RabA1a-GDP and RabA1a-GppNHp complexes were collected toresolutions of 2.8 A and 2.6 A, respectively. The crystal of the RabA1a-GDP complex belonged to the space group P21, withunit cell parameters a = 36.7, b = 70.4, c = 68.0 A, and β = 97.2°. The crystal of the RabA1a-GppNHp complex belonged tothe space group P1, with unit cell parameters, a = 37.6, b = 48.4, c = 68.5 A, α = 88.5, β = 74.1, and γ = 84.4°. Both crystalscontained two macromolecules each containing GDP or GppNHp in the asymmetric units.
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참고문헌 (19)
참고문헌 신청
Adams, P. D / 2010 / PHENIX : a comprehensive Python-based system for macromolecular structure solution / Acta Crystallogr D Biol Crystallogr 66:213~221
Asaoka, R / 2013 / Arabidopsis RABA1 GTPases are involved in transport between the trans-Golgi network and the plasma membrane, and are required for salinity stress tolerance / Plant J 73:240~249
Asaoka, R / 2013 / New insights into the role of Arabidopsis RABA1 GTPases in salinity stress tolerance / Plant Signal Behav 8:e25377
Colicelli, J / 2004 / Human RAS superfamily proteins and related GTPases / Sci STKE 2004 :RE13
Feraru, E / 2012 / BEX5/RabA1b regulates trans-Golgi network-to-plasma membrane protein trafficking in Arabidopsis / Plant Cell 24:3074~3086
Asaoka, R / 2013 / Arabidopsis RABA1 GTPases are involved in transport between the trans-Golgi network and the plasma membrane, and are required for salinity stress tolerance / Plant J 73:240~249
Asaoka, R / 2013 / New insights into the role of Arabidopsis RABA1 GTPases in salinity stress tolerance / Plant Signal Behav 8:e25377
Colicelli, J / 2004 / Human RAS superfamily proteins and related GTPases / Sci STKE 2004 :RE13
Feraru, E / 2012 / BEX5/RabA1b regulates trans-Golgi network-to-plasma membrane protein trafficking in Arabidopsis / Plant Cell 24:3074~3086
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