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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제26권 제12호
- 발행연도
- 2016.12
- 수록면
- 2,127 - 2,137 (11page)
- DOI
- 10.4014/jmb.1607.07025
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초록· 키워드
오류제보하기
A mangrove microbial community was analyzed at the gene and protein levels using metagenomic and proteomic methods with the green macroalgae Enteromorpha prolifera as the substrate. Total DNA was sequenced on the Illumina HiSeq 2000 PE-100 platform. Twodimensional gel electrophoresis in combination with liquid chromatography tandem mass spectrometry was used for proteomic analysis. The metagenomic data revealed that the orders Pseudomonadales, Rhizobiales, and Sphingomonadales were the most prevalent in the mangrove microbial community. By monitoring changes at the functional level, proteomic analyses detected ATP synthase and transporter proteins, which were expressed mainly by members of the phyla Proteobacteria and Bacteroidetes. Members of the phylum Proteobacteria expressed a high number of sugar transporters and demonstrated specialized and efficient digestion of various glycans. A few glycoside hydrolases were detected in members of the phylum Firmicutes, which appeared to be the main cellulose-degrading bacteria. This is the first report of multiple “omics” analysis of E. prolifera degradation. These results support the fact that key enzymes of glycoside hydrolase family were expressed in large quantities, indicating the high metabolic activity of the community.
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참고문헌 (54)
참고문헌 신청
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Balat M / 2011 / Production of bioethanol from lignocellulosic materials via the biochemical pathway: a review / Energy Convers. Manage 52:858~875
Barnard D / 2010 / Extremophiles in biofuel synthesis / Environ. Technol 31:871~888
Benson D / 2006 / GenBank / Nucleic Acids Res 34:D16~D20
Bradford MM / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72:248~254
Balat M / 2011 / Production of bioethanol from lignocellulosic materials via the biochemical pathway: a review / Energy Convers. Manage 52:858~875
Barnard D / 2010 / Extremophiles in biofuel synthesis / Environ. Technol 31:871~888
Benson D / 2006 / GenBank / Nucleic Acids Res 34:D16~D20
Bradford MM / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72:248~254
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