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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.12
- 수록면
- 432 - 437 (6page)
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Interleukin-11 (IL-11) is a cytokine that plays a key regulatory role in the immune system. Recombinant human IL-11 (rhIL-11) exerts a preventative effect against apoptotic cell death and inhibits preadipocyte differentiation. IL-11 also is used to stimulate the bone marrow to produce platelets in order to prevent low platelets that may be caused by chemotherapy. Unfortunately, the high production cost of IL-11 associated. In this study, we investigated the feasibility of transgenic plants for the cost-effective production of rhIL-11. Production of rhIL-11 proteins in whole-plant expression system will be more economical when compared to the current E. coli based expression system. The human rhIL-11 gene was codon optimized to maximize plant host system expression. IL-11 expression vector under the control of a constitutive cauliflower mosaic virus 35S (CaMV 35S) promoter was introduced into tobacco by Agrobacterium- mediated transformation. The 5′-leader sequence (called Ω) of tobacco mosaic virus (TMV) as a translational enhancer was added to construct. Transgenic tobacco plants expressing various levels of rhIL-11 protein were generated. Western blotting of the stably transformed lines demonstrated accumulation of the appropriately sized rhIL-11 protein in leaves. This research demonstrated the efficacy of using tobacco as an expression system for the production of rhIL-11.
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참고문헌 (20)
참고문헌 신청
Bradford MM / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal Biochem 72:248~254
Cerutti H / 1997 / Epigenetic silencing of a foreign gene in nuclear transformants of Chlamydomonas / Plant Cell 9:925~945
Creager ANH / 1999 / Tobacco mosaic virus: pioneering research for a century / Plant Cell 11:301~308
Cui J / 2008 / Expression of Recombinant Human Interleukin-2 in Tomato Fruits / J Biotech 136:S235~S235
Gallie DR / 1987 / The 5′-leader sequence of tobacco mosaic virus RNA enhances the expression of foreign gene transcripts in vitro and in vivo / Nucleic Acids Res 15:3257~3273
Cerutti H / 1997 / Epigenetic silencing of a foreign gene in nuclear transformants of Chlamydomonas / Plant Cell 9:925~945
Creager ANH / 1999 / Tobacco mosaic virus: pioneering research for a century / Plant Cell 11:301~308
Cui J / 2008 / Expression of Recombinant Human Interleukin-2 in Tomato Fruits / J Biotech 136:S235~S235
Gallie DR / 1987 / The 5′-leader sequence of tobacco mosaic virus RNA enhances the expression of foreign gene transcripts in vitro and in vivo / Nucleic Acids Res 15:3257~3273
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