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논문 기본 정보

자료유형
학술저널
저자정보
최병훈 (국립부경대학교 4차산업융합바이오닉스공학과) 조성한 (국립부경대학교 4차산업융합바이오닉스공학과) 박상혁 (국립부경대학교 4차산업융합바이오닉스공학과 2 국립부경대학교 스마트헬스케어학부 스마트헬스케어학부 의공학전공)
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대한의용생체공학회 의공학회지 의공학회지 제45권 제1호
발행연도
2024.2
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20 - 25 (6page)

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초록· 키워드

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Extracellular vesicles (EVs) are nano-sized lipid bilayer vesicles released by cells. EVs act as messengers for cell-to-cell communication. Inside, it contains various substances that show biological activity, such as proteins, lipids, nucleic acids, and metabolites. The study of EVs extracted from terrestrial organisms and stem cells on inflam- matory environments and tissue regeneration have been actively conducted. However, marine organisms-derived EVs are limited. Therefore, we have extracted EVs from sea urchins belonging to the Echinoderm group with their excellent regenerative ability. First, we extracted extracellular matrix (ECM) from sea urchin gonads treated with hypotonic buffer, followed by collagenase treatment, and filtration to collect ECM-bounded EVs. The size of sea ur- chin gonad-derived EVs (UGEVs) is about 20-100 nm and has a round shape. The protein content was higher after EVs burst than before, which is evidence that proteins are contained inside. In addition, proteins of various sizes are distributed inside. PKH-26 was combined with UGEVs, which means that UGEVs have a lipid membrane. PHK-26- labeled UGEVs were successfully uptaken by cells. UGEVs can be confirmed to have the same characteristics as tra- ditional EVs. Finally, it was confirmed that Schwann cells were not toxic by increasing proliferation after treatment.

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