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    초록·키워드

    The bacterial enhancer-binding protein nitrogen regulatory protein C (NtrC) activates transcription by the σ⁵⁴-holoenzyme form of RNA polymerase in a reaction that depends upon hydrolysis of ATP. Phosphorylation of an aspartate residue in the N-terminal receiver domain of NtrC induces oligomerization of the protein and activates the ATPase activity. To study communication between the receiver domain of NtrC, which is known to act positively, and its output domain, we isolated mutant forms of the protein carrying single cysteine residues and derivatized them with a sulfhydryl-specific nitroxide reagent for electron paramagnetic resonance spectroscopic studies of nitroxide mobility. The single cysteines were placed at four positions at which we had obtained constitutive amino acid substit ... 전체 초록 보기

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