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DBpia AI
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Citeasy
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초록·키워드
Mercury is a major inorganic contaminant in water due to its high health hazards. In the last three decades, there has been an increasing awareness of mercury toxicity in various aquatic environments. Mercury undergoes a wide variety of chemical and biological transformations in aquatic ecosystems, and its fate highly depends on microbe-mediated transformations. Mercury biotoxicity is subject to its speciation and uptake by microbial cells. Like other metals, species of mercury can alter after chelating substances from organic and inorganic ligands. Dissolved organic matter (DOM) and ethylenediaminetetraacetic acid (EDTA) have been reported to be the most important organic ligands for aqueous mercury. However, arguments exist concerning the role of humic acids in determining the interactions between mercury and organism surfaces. Decrease, increase, and no effect of mercury uptake due to DOM have all been reported. An effective approach to measuring mercury bioavailability and evaluating the ligand impact on mercury uptake is biosensing technique, which could reflect the real physiological impact of toxic compounds. This study examined the kinetic response of a lux-based engineered bacterial bioreporter (strain E. Coli ARL1) to mercury under different environmental conditions, such as solution pH and presence of HA and EDTA. Our results indicate that the ARL1 bioreporter can detect Hg(II) at concentrations as low as 0.5 μg/L and there is an excellent nonlinear relationship between bioluminescence emission and mercury concentration up to 2,500 μg/L. The ARL1 concentration for the best response was found to be ~108 cfu/L. As for ligand impact, a gradual increase in light emission was observed with increasing concentrations of HA (as natural source ligands), su ... 전체 초록 보기
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