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(Sogang University) (Sogang University) (Yonsei University College of Medicine) (Yonsei University College of Medicine) (Yonsei University College of Medicine) (Sogang University)
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한국미생물학회 한국미생물학회 학술대회논문집 2014년도 International Meeting of the Microbiological Society of Korea
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    초록·키워드

    Vibrio vulnificus, a causative agent of septicemia and gastroenteritis, secretes various proteins, which are assumed to be involved in various functional types of interactions with hosts during infection process. Protease-zymogram analyses revealed the presence of multiple extracellular proteases in the supernatant cultured by V. vulnificus. One of them is a putative metalloprotease containing a Zn-binding motif, whose proteolytic activity was abolished by specific inhibitors against metalloproteases. Thus, it was named by VvpM to distinguish from VvpE that has been considered as a representative extracellular protease of V. vulnificus. To investigate whether this newly identified protease, VvpM has pathogenic role in host interaction in addition to proteolytic role, several human cell lines were incubated in the presence of rVvpM. VvpM-challenged cells showed typical apoptosis characterized by cell shrinkage, morphological changes in nucleus, and appearance of vacuoles in cytoplasm. Apoptotic cell death was further evidenced by estimating the ... 전체 초록 보기

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      UCI(KEPA) : I410-ECN-0101-2017-475-001053489