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논문 기본 정보

저자정보
(영남대학교) (Washington State University) (Washington State University)
저널정보
대한기계학회 대한기계학회 춘추학술대회 대한기계학회 2008년도 추계학술대회 논문집(경북지구)
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    초록·키워드

    Ampholyte based isoelectric focusing (IEF) simulation was conducted to study dispersion of proteins in a horse shoe microchannel. Four model proteins (pls= 6.49, 7.1, 7.93 and 8.6) are focused in a 1 ㎝ long horse shoe channel under an electric field of 300 V/㎝. The pH gradient is formed in the presence of 25 biprotic carrier ampholytes (?pK = 3.0) within a pH range of 6 to 9. The proteins are focused at 380 sec in a nominal electric field of 300 V/㎝. Our numerical results show that the band dispersions of a protein are large during the gradient formation stage, but the dispersions are significantly reduced when the double peaks start to merge. This rearrangement of spreading band isvery unique compared to linear electrokinetic phenomena (capillary zone electrophoresis) and is independent of channel position and channel shape. Hence, one can perform IEF and other self-sharpening electrokinetic methods, such as, isotachophoresis (ITP), in complex geometries without incorporating hyperturns.

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      UCI(KEPA) : I410-ECN-0101-2010-550-002815114