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    We describe a new method for the detection of conformational change based on reconstitution of split green fluorescent protein (GFP). In this study, we used fluorescence complementation assay, which was designated (FCA), for monitoring conformationally changed protein. Particularly, we used maltose binding protein (MBP) as a model protein, as MBP undergoes its hinge-twist movement upon binding of the substrate. The common feature of this approaches that GFP as a reporter protein is split into two non-fluorescent fragments, which are genetically fused to N- and C-termini of MBP. Upon the binding of maltose, chrom ... 전체 초록 보기

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