지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.12
- 수록면
- 80 - 86 (7page)
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초록· 키워드
오류제보하기
Purpose: Current common method of cytotoxicity evaluation for elastomeric impression materials use animal based cell lines, which the clinical relevance has been often questioned. Hence, the purpose of this study was to examine the difference in results with both human based and animal based fibroblast cell line.
Materials and Methods: Three types of fibroblast cells were used in this study; conventional mouse fibroblasts of L929, human gingival fibroblasts (HGF-1), and immortalized human oral fibrobalsts (hTERT-hNOF). Test on extract and test by direct contact using different commercially available elastomeric impression materials were carried out according to the international standards.
Result: There was significant difference in cell viability between types of fibroblasts cell used, where HGF-1 showed highest cell viability and L929 the lowest.
Conclusion: Within the limitation of this study, careful consideration must be given when selecting the cells and interpreting the results for cytotoxicity evaluation of elastomeric impression materials, where use of human based cell lines such as hTERT-hNOF would be appropriate for both ease of cytotoxicity test and clinical relevance.
Materials and Methods: Three types of fibroblast cells were used in this study; conventional mouse fibroblasts of L929, human gingival fibroblasts (HGF-1), and immortalized human oral fibrobalsts (hTERT-hNOF). Test on extract and test by direct contact using different commercially available elastomeric impression materials were carried out according to the international standards.
Result: There was significant difference in cell viability between types of fibroblasts cell used, where HGF-1 showed highest cell viability and L929 the lowest.
Conclusion: Within the limitation of this study, careful consideration must be given when selecting the cells and interpreting the results for cytotoxicity evaluation of elastomeric impression materials, where use of human based cell lines such as hTERT-hNOF would be appropriate for both ease of cytotoxicity test and clinical relevance.
목차
Introduction
Materials and Methods
Result
Discussion
Conclusion
References
참고문헌 (22)
참고문헌 신청
1. [학술지(정기간행물)] - Brown D / 1996 / Dental materials: 1994 literature review / J Dent 24 : 153 ~ 184
2. [학술지(정기간행물)] - Coppi C / 2007 / A new generation of sterile and radiopaque impression materials: an in vitro cytotoxicity study / J Biomater Appl 22 : 83 ~ 95
3. [학술지(정기간행물)] - Dahl BL / 1990 / Assessment of adverse reactions to prosthodontic materials / J Oral Rehabil 17 : 279 ~ 286
4. [학술지(정기간행물)] - Wataha JC / 2001 / Principles of biocompatibility for dental practitioners / J Prosthet Dent 86 : 203 ~ 209
5. [학술지(정기간행물)] - Vajrabhaya L / 1997 / Multilayer and monolayer cell cultures in a cytotoxicity assay of root canal sealers / Int Endod J 30 : 141 ~ 144
2. [학술지(정기간행물)] - Coppi C / 2007 / A new generation of sterile and radiopaque impression materials: an in vitro cytotoxicity study / J Biomater Appl 22 : 83 ~ 95
3. [학술지(정기간행물)] - Dahl BL / 1990 / Assessment of adverse reactions to prosthodontic materials / J Oral Rehabil 17 : 279 ~ 286
4. [학술지(정기간행물)] - Wataha JC / 2001 / Principles of biocompatibility for dental practitioners / J Prosthet Dent 86 : 203 ~ 209
5. [학술지(정기간행물)] - Vajrabhaya L / 1997 / Multilayer and monolayer cell cultures in a cytotoxicity assay of root canal sealers / Int Endod J 30 : 141 ~ 144
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UCI(KEPA) : I410-ECN-0101-2016-515-001062003