Safety assessment of the AtCYP78A7 protein expressed in genetically modified rice tolerant to abiotic stress

Q: What is AtCYP78A7 protein as defined in this paper?

AtCYP78A7 protein encodes a cytochrome P450 gene derived from Arabidopsis thaliana and is overexpressed in environment-stress-tolerant GM rice to enhance drought stress tolerance and increase seed size. This protein consists of 536 amino acids and underwent amino acid sequence homology analysis and acute oral toxicity tests to evaluate its potential allergenicity and toxicity.

Q: How was the safety of the AtCYP78A7 protein expressed in GM rice evaluated?

The research findings indicate that the AtCYP78A7 protein exhibited no amino acid sequence homology with known allergens or protein toxins. Furthermore, no toxicological changes were observed in mortality, body weight changes, clinical symptoms, or necropsy findings when a single oral dose of 2,000 mg/kg was administered to mice. Therefore, it is concluded that the AtCYP78A7 protein poses a very low risk of allergenicity and acute toxicity.

Kyong-Hee Nam; Do Young Kim; Hee Jae Shin; In-Soon Pack; Jung-Ho Park; Won Kee Yoon; Ho Bang Kim; Chang-Gi Kim

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Type: Academic journal

Author: Kyong-Hee Nam (KRIBB) Do Young Kim (KRIBB) Hee Jae Shin (KRIBB) In-Soon Pack (KRIBB) Jung-Ho Park (KRIBB) Won Kee Yoon (KRIBB) Ho Bang Kim (Biomedic) Chang-Gi Kim (KRIBB)

Journal: Institute of Agricultural Science Korean Journal of Agricultural Science Vol.45 No.2

Published: 2018.6

Pages: 248 - 257 (10page)

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Overexpression of AtCYP78A7, a gene encoding a cytochrome P450 protein, has been reported to improve tolerance to drought stress in genetically modified (GM) rice (Oryza sativa L.). The aim of this study was to evaluate the potential allergenicity and acute oral toxicity of the AtCYP78A7 protein expressed in GM rice. Bioinformatics analysis of the amino acid sequence of AtCYP78A7 did not identify any similarities with any known allergens or toxins. It showed that no known allergen had more than a 35% amino acid sequence homology with the AtCYP78A7 protein over an 80 amino acid window or more than 8 consecutive identical amino acids. The gene encoding the AtCYP78A7 protein was cloned in the pGEX-4T-1 vector and expressed in E. coli . Then, the AtCYP78A7 protein was purified and analyzed for acute oral toxicity. The AtCYP78A7 protein was fed at a dose of 2,000 ㎎/㎏ body weight in mice, and the changes in mortalities, clinical findings, and body weight were monitored for 14 days after the dosing. Necropsy was carried out on day 14. The protein did not cause any adverse effects when it was orally administered to mice at 2000 ㎎/㎏ body weight. These results indicate that the AtCYP78A7 protein expressed in GM rice would not be a potential allergen or toxin.

#acute oral toxicity #allergenicity #cytochrome P450 #genetically modified crop #rice #safety assessment

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