지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.9
- 수록면
- 173 - 177 (5page)
- DOI
- 10.7841/ksbbj.2019.34.3.173
이용수
초록· 키워드
오류제보하기
A sequential statistical optimization was applied to the components of Tris-acetate-phosphate (TAP) medium, to enhance the cell density and lipid production of Arctic microalga Chlamydomonas sp. KNM0029C. The evaluation was preformed through an elimination process and Plackett-Burman design to select for significant factors, contributing toward cell growth. NH₄Cl, MgSO₄·7H₂O, potassium phosphate, and trace elements were established as the effective components and used as variable factors in Box-Behnken design test, - a response surface methodology (RSM). The final optimized concentrations were 0.54, 0.16, 0.04, and 0.08 g/L of NH₄Cl, MgSO₄·7H₂O, potassium phosphate, and trace elements, respectively. Overall, 9.9% enhanced lipid production was achieved by using optimized TAP medium at 4°C. The results of the present study could potentially contribute toward large-scale lipid production at low temperatures.
목차
Abstract
1. INTRODUCTION
2. MATERIALS AND METHODS
3. RESULTS AND DISCUSSION
4. CONCLUSION
REFERENCES
참고문헌 (11)
참고문헌 신청
Gerday, C. / 2000 / Cold-adapted enzymes: from fundamentals to biotechnology / Trends Biotechnol 18:103~107
Kim, S. / 2015 / Enhancing extracellular lipolytic enzyme production in an Arctic bacterium, Psychrobacter sp. ArcL13, by using statistical optimization and fed-batch fermentation / Prep. Biochem. Biotechnol 45:348~364
Lee, J. H. / 2013 / Optimization of the pilot-scale production of an ice-binding protein by fed-batch culture of Pichia pastoris / Appl. Microbiol. Biotechnol 97:3383~3393
Kim, E. J. / 2016 / Growth and lipid content at low temperature of Arctic alga Chlamydomonas sp. KNM0029C / Bioproc. Biosyst. Eng 39:151~157
Govarthanan, M. / 2014 / Statistical optimization of alkaline protease production from brackish environment Bacillus sp. SKK11 by SSF using horse gram husk / Prep. Biochem. Biotechnol 44:119~131
Kim, S. / 2015 / Enhancing extracellular lipolytic enzyme production in an Arctic bacterium, Psychrobacter sp. ArcL13, by using statistical optimization and fed-batch fermentation / Prep. Biochem. Biotechnol 45:348~364
Lee, J. H. / 2013 / Optimization of the pilot-scale production of an ice-binding protein by fed-batch culture of Pichia pastoris / Appl. Microbiol. Biotechnol 97:3383~3393
Kim, E. J. / 2016 / Growth and lipid content at low temperature of Arctic alga Chlamydomonas sp. KNM0029C / Bioproc. Biosyst. Eng 39:151~157
Govarthanan, M. / 2014 / Statistical optimization of alkaline protease production from brackish environment Bacillus sp. SKK11 by SSF using horse gram husk / Prep. Biochem. Biotechnol 44:119~131
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UCI(KEPA) : I410-ECN-0101-2019-470-001230318