인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
개인구독
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지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
In this study, we developed and validated a high-performance liquid chromatography/diode array method for the quantification of Hyperoside and quercetin-3-glucoside as a marker compound in water dropworth (Oenanthe javanica DC.) extract (WDE). Method validation was performed by measuring specificity, linearity, limit of detection (LOD), limit of quantitation (LOQ), accuracy, and precision. Hyperoside and quercetin-3-glucoside were separated without interference from other substances at the same retention time and spectrum as the standard solution, demonstrating the analytical specificity. Calibration curves of hyperoside and quercetin-3-glucoside exhibited high linearity (R² ≥1.000). The LOD and LOQ of hyperoside and quercetin-3-glucoside were 0.16, 0.49 μg/mL and 0.14, 0.41 μg/mL, respectively. The recovery rate of hyperoside and quercetin-3-glucoside were obtained 97.20–99.78% and 99.44–104.37%, respectively, indicating that the results were highly accuracy. Relative standard deviation values for precision of hyperoside and quercetin-3-glucoside were 0.84-1.62% and 1.96-2.50%, respectively. These results indicate that the proposed HPLC/DAD method for the detection of hyperoside and quercetin-3-glucoside in WDE is simple, reliable, and reproducible. Therefore, this analytical method could be used as basic data for the development of functional ingredients for health functional food of WDE.
본문·목차
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UCI(KEPA) : I410-151-25-02-092186670