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논문 기본 정보

자료유형
학술저널
저자정보
저널정보
한국식품과학회 Food Science and Biotechnology Food Science and Biotechnology vol 14. no 2
발행연도
수록면
263 - 267 (5page)

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초록· 키워드

In order to isolate hyaluronidase (HAase) inhibitor from Astragali radix (AR), dried roots were extracted with ethanol, prior to sequential fractionations with n-hexane, chloroform, ethyl acetate, n-butanol, and aqueous fractions. The n-butanol soluble fraction was found to exhibit the most pronounced inhibitory effect (68%) on HAase, and the active components were separated using various chromatographic methods, including column chromatography and preparative HPLC. The active component was isolated from the n-butanol soluble fraction of AR and was structurally identified as calycosin-7-O-β-D-glucopyranoside by LC-MS, IR, 1H NMR, and 13C NMR analysis. The IC50 of calycosin-7-O-β-D-glucopyranoside’s HAase activity was found to be 3.7 mg/mL.
#Astragalus membranaceus Bunge #calycosin-7-o-β-D-glucopyronoside #hyaluronidase inhibitor
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목차

  1. Abstract
  2. Introduction
  3. Materials and Methods
  4. Results and Discussion
  5. Acknowledgements
  6. References

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UCI(KEPA) : I410-ECN-0101-2013-573-001294547