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논문 기본 정보

자료유형
학술저널
저자정보
(Seoul National University) (Seoul National University) (Seoul National University)
저널정보
한국식품과학회 Food Science and Biotechnology Food Science and Biotechnology vol 16. no 5
발행연도
수록면
778 - 782 (5page)

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초록· 키워드

This study assessed the effects of the antimicrobial substances sulforaphane, grapefruit seed extracts (GSE), and reuterin on the expression of Salmonella HilA and InvF virulence gene using a LacZY assay (β-galactosidase assay) with hilA:lacZY and invF:lacZY fusion strains of Salmonella typhimurium SL1344. Salmonella was grown for 8 hr at 37oC in the presence of diluted antimicrobial substances (2 μg/mL sulforaphane, 20 μg/mL GSE, and 0.26 mM reuterin) at concentrations that did not inhibit the cellular growth of Salmonella. Sulforaphane inhibited the expression of HilA and InvF by 50-90 and 20-80%, respectively. GSE also inhibited the expression of both genes, but to a lesser degree. Among the 3 antimicrobial substances, reuterin showed the least inhibition, which was abolished after 3-4 hr. None of the antimicrobial substances inhibited the β-galactosidase enzyme activity of S. typhimurium. The assay used in this study represents a very sensitive method for screening bioactive substances that inhibit the expression of virulence genes in Salmonella.
#virulence expression #antimicrobial substance #hilA #invF
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목차

  1. Abstract
  2. Introduction
  3. Materials and Methods
  4. Results and Discussion
  5. Acknowledgments
  6. References

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UCI(KEPA) : I410-ECN-0101-2013-573-001011226