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초록·키워드
Background: In the present study, we identified two cytotoxic compounds from the root of Rumex crispus L. using a bioassaybased method.
Methods and Results: Compared with the other fractions, the diethyl ether (Et₂O) fraction of R. crispus root extract exhibited the strongest of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging effect [scavenging concentration 50% (SC<SUB>50</SUB>) = 63.8 ± 1.47 ㎍/㎖], nitric oxide (NO) production inhibitory effect on the mouse macrophage cell line RAW264.7 [inhibitory concentration 50% (IC50) = 60.9 ± 7.52 ㎍/㎖] and cytotoxicity effect on the human hepatoma cell line, HepG2 [lethal concentration 50% (LC<SUB>50</SUB>) = 115.4 ± 1.86 ㎍/㎖]. According to the bioassay-based method, two cytotoxic compounds were purified from the Et₂O fraction by using column chromatography and preparative high performance liquid chromatography (prep-HPLC). These two compounds were identified as parietin and chrysophanol by using nuclear magnetic resonance (NMR) and liquid chromatography quadruple time of flight mass spectrometry (LC-QTOF-MS). In addition, both parietin and chrysophanol exhibited a cytotoxicity effect on HepG2 cells, their LC<SUB>50</SUB> values were 169.1 ± 17.67 μM and 111.5 ± 6.62 μM, respectively.
Conclusions: Parietin and chrysophanol isolated from the Et₂O fraction of the R. crispus root extract showed cytotoxicity in HepG2 cell.
Methods and Results: Compared with the other fractions, the diethyl ether (Et₂O) fraction of R. crispus root extract exhibited the strongest of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging effect [scavenging concentration 50% (SC<SUB>50</SUB>) = 63.8 ± 1.47 ㎍/㎖], nitric oxide (NO) production inhibitory effect on the mouse macrophage cell line RAW264.7 [inhibitory concentration 50% (IC50) = 60.9 ± 7.52 ㎍/㎖] and cytotoxicity effect on the human hepatoma cell line, HepG2 [lethal concentration 50% (LC<SUB>50</SUB>) = 115.4 ± 1.86 ㎍/㎖]. According to the bioassay-based method, two cytotoxic compounds were purified from the Et₂O fraction by using column chromatography and preparative high performance liquid chromatography (prep-HPLC). These two compounds were identified as parietin and chrysophanol by using nuclear magnetic resonance (NMR) and liquid chromatography quadruple time of flight mass spectrometry (LC-QTOF-MS). In addition, both parietin and chrysophanol exhibited a cytotoxicity effect on HepG2 cells, their LC<SUB>50</SUB> values were 169.1 ± 17.67 μM and 111.5 ± 6.62 μM, respectively.
Conclusions: Parietin and chrysophanol isolated from the Et₂O fraction of the R. crispus root extract showed cytotoxicity in HepG2 cell.
본문·목차
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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UCI(KEPA) : I410-ECN-0101-2019-480-000930953
