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한국생물공학회 KSBB Journal KSBB Journal 제16권 제4호
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    초록·키워드

    Catechin compounds from the Green tea cultivated in Bosung (Chollanamdo) were extracted using supercritical fluid and various additives, and analyzed by reversed-phase high performance liquid chromatography (RP-HPLC). The chromatographic column was packed with LiChrospher 100RP-18(15 μm), and water was used as the mobile phase with 0.05% phosphoric acid and acetonitrile. Gradient elution was applied to separate EGCG by changing the mobile phase compositions. Comparing the extraction yield of three different types of supercritical fluids, pure CO₂, with additives of water and ethanol (5 wt.%), the extraction amount of EGCG was most abundant in the supercritical CO₂ with ethanol. However, more was extracted and pure higher purity was achieved by solvent extraction using ethanol.

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