This study was performed to investigate the bioactivity of Ti-24Nb-17Ta-5Zr alloy modified by an anodic oxidation and an precalcification treatments. Ti-27Nb-10Ta-3.5Zr alloy was anodized to form the nanotubular TiO₂ layer. To increase the bioactivity, calcium phosphate deposition was carried out by an immersion treatment in 0.5 M CaHPO₄ solution at 90℃ for 30 min. Bioactivities were evaluated by the apatite forming ability in simulated body fluid (SBF) for 10 days. Nanotubular TiO₂ layer showed the dense and self-organized structure. Nanotubes showed the separately connected tube structures and it was opened and enlarged as going down. The bioactivity of nanotubular TiO₂ layer on Ti-27Nb-10Ta-3.5Zr alloy was improved with precalcification treatment in 0.5 M CaHPO₄ at 90℃. These results suggest that the bioactivity of Ti-27Nb-10Ta-3.5Zr alloy could be improved with the formation of nanotubular TiO₂ layer and the preecalcification treatment in 90℃ 0.5 M CaHPO₄ solution.