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(Kunsan National University) (Kunsan National University) (Kunsan National University) (Kunsan National University) (Kunsan National University) (Kunsan National University) (Kunsan National University) (Kunsan National University) (Kunsan National University)
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한국수산과학회 양식분과 한국수산과학회 양식분과 학술대회 2016 한국수산과학회 춘계학술대회
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    초록·키워드

    An approximately 6.4 kDa (6483.48 Da) antimicrobial polypeptide, cgLR29, was purified from the acidified gill extract of the Pacific Oyster, Crassostrea gigas, by cation-exchange and C18 RP-HPLC. The extraction was performed with acidic water containing 1% acetic acid [1 (tissue):4 (1% HAc) volume ratio]. The acidified gill extract showed potent antimicrobial activities against B. subtilis and E. coli D31 without significant hemolytic activity and its activity was trypsin-dependent. To purify antimicrobial polypeptide, the extract was first fractionated through cation-exchange HPLC and then subsequently the fractions were loaded into C18 RP-HPLC. The active polypeptide was finally purified to homogeneity with the same C₁₈ column and was chara ... 전체 초록 보기

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      UCI(KEPA) : I410-ECN-0101-2017-529-000779430