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(Pukyong National University) (Pukyong National University) (Pukyong National University) (Pukyong National University) (Pukyong National University) (Pukyong National University) (Pukyong National University)
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한국생물공학회 한국생물공학회 학술대회 2009 춘계학술대회 및 국제심포지움
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    Rapamycin is a macrocyclic polyketide with immunosuppressive, antifungal, and anticancer activity. The production level of rapamycin in Streptomyces hygroscopicus ATCC29253 was enhanced by classical mutagenesis and metabolic engineering approach. This study describes the development of a novel high-throughput method for rapid screening of rapamycin-producing strains using agar diffusion method and metabolic engineering strategy to enhanced rapamycin production. Rapamycin production by a mutant strain UV2-2 induced by Ultraviolet (UV) mutagenesis was improved by approximately 3.2-fold compared to that of wild-type strain. The comparative analysis of gene expression and intracellular acyl-CoA pool between ... 전체 초록 보기

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