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논문 기본 정보

자료유형
학술저널
저자정보
(National Academy of Agricultural Science) (Institute for Future Environmental Ecology) (ChunLab) (National Academy of Agricultural Science) (National Academy of Agricultural Science) (National Academy of Agricultural Science) (National Academy of Agricultural Science) (National Academy of Agricultural Science) (National Academy of Agricultural Science)
저널정보
한국토양비료학회 한국토양비료학회지 한국토양비료학회지 제48권 제5호
발행연도
수록면
442 - 450 (9page)

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초록· 키워드

This study was conducted to investigate the effect of Psy-2A-CrtI (PAC), a genetically modified (GM) rice with enhanced β-carotene, on the soil microbial community. The soil used to cultivate GM rice and its wild-type, Nakdong, was analyzed for population density, denaturing gradient gel electrophoresis (DGGE), and pyrosequencing. It was found that the bacterial, fungal and actinomycetes population densities of the PAC soils were within the range of those of the non-GM rice cultivar, Nakdong. The DGGE banding patterns of the GM and non-GM soils were also similar, suggesting that the bacterial community structures were stable within a given month and were unaffected by the presence of a GM plant. The pyrosequencing result showed a temporal difference in microorganism taxon and distribution ratio, but no significant difference between GM and non-GM was found. The persistence of the transgene DNA in the plant and surrounding soil were investigated for different time periods. There were differences in the persistence within the plant depending on the gene, but they could not be detected after 5 weeks. Also the transgenes were not detected in the surrounding soil. These results indicate that soil microbial communities are unaffected by the cultivation of a PAC rice within the experimental time frame.
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목차

  1. Introduction
  2. Materials and Methods
  3. Results and Discussion
  4. Conclusion
  5. References

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UCI(KEPA) : I410-ECN-0101-2016-521-002012994