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논문 기본 정보

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학술대회자료
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대한환경공학회 대한환경공학회 학술발표논문집 대한환경공학회 1998년도 추계학술발표논문집
발행연도
수록면
3 - 9 (7page)

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초록· 키워드

There are many examples of environmental engineering system, where the structure and activity of microbial ecological system plays a crucial role. To monitor and control the microbial ecosystems used in environmental engineering the identification of the components of microbial community is an essential procedure. The routine bacteriological isolation of pure cultures and their further identification are not suitable for environmental engineering because traditional cytological, biochemical, physiological, and molecular biological identification is usually the long-term and expensive techniques. Most suitable approach for microbiological monitoring of environmental engineering systems is the use of RNA-targeted probes. The method of whole cell fluorescent in situ hybridization (FISH) with rRNA-targeted, fluorescent oligonucleotide probes was used successfully to analyze the bacterial communities of different environmental engineering systems. However, specific probes are expensive and cannot be used in environmental engineering for simultaneous rapid identification of the cells within the bacterial community. Another way for molecular identification of bacterial taxon in environmental engineering systems was discussed in this paper. It is the technique called as non-specific FISH. The method is based on data that the combination of the numbers of different nucleotide triplets in 16S RNA is species-specific or genus-specific character. Therefore, using non-specific FISH with three different labeled triplets the environmental engineer may, probably, distinguish and identify many bacterial specia or genera simultaneously. Flow cytofluorometer or confocal laser microscope with the software for image analysis is most suitable equipment for the identification by non-specific FISH. Simplest modification of non-specific FISH identification is fluorescent spectrometry of isolated pure bacterial culture hybridizing with labeled triplets.
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UCI(KEPA) : I410-ECN-0101-2017-539-001486907